摘要
目的 :1995年山西晋南地区发生恙虫病流行 ,我们对该地区人群进行了流行病学调查 ;并对从患者和野鼠体内分离的恙虫病立克次体进行了病原学研究。方法 :利用微量间接免疫荧光法(MIF)对恙虫病立克次体在人群中的流行进行了调查 ;采用MIF、PCR/RFLP和序列分析方法对恙虫病立克次体分离株进行了鉴定。结果和结论 :晋南地区人群血清恙虫病立克次体抗体阳性率为2 5% ;恙虫病立克次体山西分离株 (Sxh951,Sxh952 ,Sxh953,Sxm97)的血清型别为Gilliam株 ;序列分析提示 :在相对分子质量 560 0 0蛋白基因水平上 ,Sxh951株与Yongchon株亲缘关系最近。
Objective: To investigate the epidemiology of scrub typhus in people of Shanxi province during an epidemic in this area in 1995 ,and to characterize the 4 Rickettsia tsutsugamushi strains isolated(Sxh951,Sxh952,Sxh953,Sxm97).Methods: Microimmuno fluoresence method was used to investigate the epidemic of R.tsutsugamushi and microimmuno fluoresence, PCR/RFLP and sequence analysis were performed to characterize these isolates. Results and conclusion: The positive rate of antibodies against R.tsutsugamushi in people of this area was 25%. The serotype of these isolates was Gilliam strain and sequence analysis suggested that in respect of M r 56 000 protein gene Sxh951 strain was closest to Yongchon strain in homology, which was isolated from South Korea. [
出处
《军事医学科学院院刊》
CSCD
北大核心
2000年第4期275-277,281,共4页
Bulletin of the Academy of Military Medical Sciences
关键词
恙虫病
流行病学
序列分析
病原学
调查
山西
scrub typhus
epidemiology
fluoroimmunoassay
serology
sequence analysis
