摘要
目的克隆恙虫病立克次体Ｇｉｌｉａｍ株５６－ｋＤａ蛋白基因，作为优化ＰＣＲ反应时的标准模板和为Ｇｉｌｉａｍ株５６－ｋＤａ蛋白基因的表达作准备。方法利用ＰＣＲ反应和Ｔ载体分别扩增和克隆目的基因片段，并用序列分析方法鉴定克隆基因的正确性。结果克隆的基因为恙虫病立克次体Ｇｉｌｉａｍ株５６－ｋＤａ蛋白基因。结论利用ＰＣＲ反应和Ｔ载体可以从少量的标本中，高效获得高纯度的目的基因。
Aim In order to optimize the condition for PCR and prepare for expression of 56 kDa protein,the gene encoding 56 kDa protein of Orientia tsutsugamushi Gilliam was cloned and two ends were sequenced Methods PCR and T vector were used to amplify and clone 56 kDa protein gene,respectively Sequence analysis was performed to identify the cloned gene Result The gene encoding 56 kDa protein of Gilliam was cloned Conclusion High pure gene fragments could be obtained from little amount of sample through PCR and T vector
出处
《中国人兽共患病杂志》
CSCD
北大核心
1999年第2期6-8,共3页
Chinese Journal of Zoonoses
