摘要
目的探讨明日叶查尔酮(AC)对2型糖尿病大鼠氧化应激水平的影响。方法雄性Wistar大鼠高脂饲料喂养加链脲佐菌素腹腔注射建立2型糖尿病模型,将造模成功大鼠随机分为糖尿病模型组、AC 30、10、5 mg/kg组及对照组,每日经口灌胃AC连续4周,检测空腹血糖、血清胰岛素、超氧化物歧化酶(SOD)、丙二醛(MDA)、总抗氧化能力、氧化型低密度脂蛋白(ox-LDL)和胰腺组织病理改变等指标。结果糖尿病模型组大鼠血糖与胰岛素水平分别为(17.30±3.57)mmol/L和(38.28±4.97)mU/L,MDA和ox-LDL含量分别为(12.00±1.32)nmol/mL和(23.05±2.87)ng/mL,均明显高于对照组(P<0.05),与糖尿病模型组比较,AC 30 mg/kg组大鼠血糖[(7.00±2.55)mmol/L]、胰岛素[(29.50±5.31)mU/L]、MDA[(9.96±0.65)nmol/mL]和ox-LDL[(15.36±1.70)ng/mL]均明显降低,差异均有统计学意义(P<0.05),胰岛细胞损伤程度减轻。结论 AC对2型糖尿病大鼠氧化应激和胰岛细胞损伤有一定抑制作用。
Objective To explore the effect of ashitaba chalcone （AC） on oxidative stress in type 2 diabetic rats. Methods Type 2 diabetic rat model was set up in male Wister rats by feeding high-fat diet and streptozotocin（STZ） in jection. Rats in AC treatment groups （at dosages of 5,10, and 30 mg/kg）were treated with gavage AC daily. Four weeks later, the fasting blood glucose （ FBG）, insulin, superoxide dismutase （ SOD ）, malondialdehyde （ MDA ）, total antioxida tive capacity（T-AOC） ,and oxidized low density lipoprotein（ox-LDL） were measured and the morphology of islet cellu lars was observed. Results In diabetic model group, the levels of FBG（ 17.30 ± 3.57 mmol/ml） ,insulin （38.28 ± 4. 97 mU/L）, MDA （12. 00 ± 1.32 nmol/ml）, and ox-LDL（23.05 ± 2. 87 ng/ml）were significantly elevated compared with those of the control group（P 〈 0. 05 for all）. In 30 mg/kg AC group,the levels of FBG （7.00±2. 55 mmol/ml） ,insu lin（29.50 ±5.31 mU/L） ,MDA （9. 96 ±0. 65 nmol/ml） ,and ox-LDL （ 15.36 ± 1.70 ng/ml） were significantly lower than those of diabetic model group （ P 〈 0. 05 for all） and the magnitude of morphological damage in islets cellular was also alleviated. Conclusion Ashitaba chalcone could inhibit oxidative stress and injury of islet cells in type 2 diabetic rats.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2014年第1期74-76,共3页
Chinese Journal of Public Health
基金
山东省医药卫生科技发展计划项目(QNW001)
