摘要
以生物转化产物9-羟基双氢青蒿素得率为响应值,对双氢青蒿素生物转化合成9-羟基双氢青蒿素培养基进行优化。在前期单因素试验的基础上,采用Plackett-Burman设计法,对影响菌株转化率的主要因素进行筛选,确定3个显著影响因素依次为玉米粉、豆粕、尿素。然后以Box-Behnken试验设计和响应面分析法,对3个显著因素进行了探讨与优化。结果显示,3个显著因素的优化水平为玉米粉30.55 g/L,豆粕7.35 g/L,尿素1.29 g/L,该条件下转化产物9-羟基双氢青蒿素得率的预测值为63.37%,实测值为64.67%,预测值与实测平均值接近,相对于培养基优化前,9-羟基双氢青蒿素得率提高了73.30%。
9-hydroxy dihydroartemisinin can be biosynthesized by the microbial transformation of dihydroartemisinin. With the 9-hydroxy dihydroartemisinin yield as response value, the biotransformation medium of dihydroartemisinin was optimized. On the basis of previous single factor experiment, Plackett-Burman design was undertaken to evaluate the effects of the seven factors. Three statistically significant parameters including corn flour, soybean meal and urea were i- dentified and optimized using the Box-Behnken design and the response surface methodology. The results showed that under the optimal conditions of corn flour 30.55 g/L, soybean meal 7.35 g/L and urea 1.29 g/L, the predicted biotrans- formation rate of 9-hydroxy dihydroartemisinin was 63.37% ,close to the experimental data of 64.67%. Through the op- timization of culture medium, the transformation yield of 9-hydroxy dihydroartemisinin increased by 73.30%.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2013年第12期1690-1695,共6页
Natural Product Research and Development
基金
2011年高等学校博士学科点专项科研基金项目(20110096130002)
江苏高校优势学科建设工程项目(PAPD)
关键词
双氢青蒿素
生物转化发酵
培养基优化
响应面分析
dihydreartemisi
biotransformation
medium optimization
response surface methodology
