内脂素在妊娠期糖尿病患者的表达及与胎儿体质量的关系被引量：4

Study on visfatin expression in gestational diabetes mellitus patients and relationship of fetal body weight.

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摘要目的:探讨妊娠期糖尿病(GDM)孕妇血、脐血及胎盘组织内脂素(visfatin)在胎儿宫内发育的作用。方法:选取行择期剖宫产终止妊娠的孕妇77例,其中GDM组30例,肥胖组20例,正常对照组27例。ELLISA法测定血清visfatin水平,荧光定量PCR及免疫蛋白印迹(western)测定胎盘组织visfatin的表达。结果:GDM组母血和脐血Visfatin水平分别为(6.72±3.79)ng/ml和(14.33±4.99)ng/ml,明显低于正常对照组(8.87±4.08)ng/ml和(24.16±9.09)ng/ml,差异有统计学意义(P<0.01);GDM组脐血Visfatin与胎盘组织visfatin mRNA表达呈正相关(P<0.05),正常组及GDM组脐血visfatin水平与新生儿PI呈负相关(P<0.01)。结论:在妊娠末期,GDM患者血清Visfatin降低参与了GDM的发生;在GDM时胎盘visfatin在胎儿宫内生长发育方面起一定作用。 Objective： To investigate visfatin concentrations in maternal/cord blood and placenta in women with gestational diabe- tes mellitus （GDM） , and to explore the effect of visfatin in the intrauterine growth of fetus. Methods： There were 77 pregnant women in the study. Thirty of these women suffered from GDM. Twenty were obese pregnant women. The remaining 27 age - matched pregnant women with normal oral glucose tolerance test served as controls. In this study, visfatin levels in maternal and cord blood were measured by ELLISA and it＇s expression in placenta was detected by PCR and Western blot. Results： The visfatin levels of maternal and cord blood were significantly lower in GDM group than the control group [ （6. 72± 3.79 ） ng/ml vs （ 8.87 ± 4. 08 ） ng/ml, P = 0. 04 ; （ 14. 33 ± 4. 99） ng/ml vs （24.16±9.09） ngc/ml, P 〈0.01 ） J ; in the GDM group, there were positively correlation in the visfatin levels of cord blood with visfatinmRNA expression in placental tissue （r = 0.58, P = 0. 04） . In the normal control group and GDM group, the visfatin levels of cord bloodwere negtively correlated with neonatal PI respectively （ r = - 0. 67, P 〈 0. 01 ; r =- - 0. 60, P 〈 0. 01 ） . Conclusion： Serum levels of visfatin are decreased in the GDM group at term. Visfatin may play a role in the GDM and intrauterine growth of fetus.

作者霍琰刘素新冯静李红艳范艳丽靳颖

机构地区河北省人民医院妇产二科

出处《中国妇幼保健》 CAS 北大核心 2014年第2期273-277,共5页 Maternal and Child Health Care of China

基金河北省科学技术研究与发展计划项目〔11276103D-25〕

关键词妊娠期糖尿病内脂素肥胖胎儿 Gestational diabetes mellitus （GDM） Visfatin Obese Fetus

分类号 R714.256 [医药卫生—妇产科学]

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参考文献26

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