摘要
目的:研究蕨菜乙醇提取物的致突变作用,为蕨菜的开发利用提供一定依据;方法:制备蕨菜乙醇提取物,采用Ames试验方法,以50μg/皿敌克松作为诱变剂,分别添加提取物50、100、200、400、800μg/皿,统计回变菌落数;结果:受试物各剂量组回变菌落数均未超过自发回变菌落数的2倍,且无剂量-反应关系;结论:在本实验条件下,蕨菜乙醇提取物无致突变作用。
Objective： To study the mutagenicity effect of ethanol extract from Pteridium aquilinum, so as to provide some help for development and utilization of the natural resource Pteridium aquilinum. Methods： To use ethanol extract from Pteridium aquilinum and adopt Ames test. Dexon in 50 Ixg/dish was chosen as a mutagen, respectively add 50, 100,200,400, 800 t^g/dish extract, and statisticalling revertant colonies. Results. The number of revertant colonies was no more than twice of that of spontane- ous colonies for each dosage group and there was no dose-response relationship existed. Conclusion： Ethanol extract from Pteridium aquilinum had no mutagenicity in this test.
出处
《中国野生植物资源》
2013年第6期7-9,共3页
Chinese Wild Plant Resources
基金
安徽省自然科学基金项目(090413113)
安徽高校省级自然科学研究重点项目(KJ2009A165)
关键词
蕨菜
乙醇提取物
AMES试验
致突变
Pteridium aquilinum
ethanol extract
Ames test
mutagenicity
