摘要
以大麦品种‘花30’为材料,用不同剂量化学诱变剂(EMS和PYM)处理大麦游离小孢子,不同剂量60Co辐照处理大麦离体穗和干种子,比较其对在低氮胁迫下小孢子培养诱导愈伤组织产量和绿苗分化数量的影响。结果表明,EMS处理离体小孢子和60Co辐照干种子明显比PYM处理小孢子和60Co辐照离体穗的培养效果好。
To investigate the effects of chemical mutagens and 60Co on callus yield and green plantlet production through microspore culture under low-nitrogen stress, we used the barley （Hordeum vulgare） variety ＇Hua 30＇ as the tested material, used two chemical mutagens, ethylmethylsulfone （EMS） and pingyangmycin （PYM）, with different concentrations to treat the microspores, and used 60C0 with different doses to treat young spikes and seeds respectively. The results showed that EMS treatment of microspores or 60Co treatment of seeds were much better than PYM treatment of microspores or 60Co treatment of young spikes for callus induction and green plantlet differentiation.
出处
《植物生理学报》
CAS
CSCD
北大核心
2013年第12期1442-1446,共5页
Plant Physiology Journal
基金
大麦青稞产业技术体系(CARS-05)
上海市种业发展项目[沪农科种字(2012)第7号]
上海市科委基础重点项目(12JC1407800)
关键词
大麦
小孢子培养
诱变
Hordeum vulgare
microspore culture
mutation
