摘要
目的 研究黄山药的化学成分 ,寻找新的活性物质。方法 用硅胶柱色谱及高效液相色谱等进行分离 ,通过理化方法及光谱分析 (IR ,ESI MS ,1HNMR ,13CNMR ,DEPT ,1H 1HCOSY ,HMQC ,HMBC ,NOESY)鉴定化学结构。结果 从黄山药根茎的乙醇提取物中分离得到 3种甾体皂苷 ,其化学结构分别鉴定为 2 6 O β D 吡喃葡糖基 3β ,2 6 二醇 2 3(S) 甲氧基 2 5 (R) Δ5,2 0 ( 2 2 ) 二烯 呋甾 3 O [α L 吡喃鼠李糖基 (1→ 2 ) O α L 吡喃鼠李糖基 (1→4) ] β D 吡喃葡糖苷 (I) ,伪原薯蓣皂苷 (pseudoprotodioscin ,II) ,2 6 O β D 葡吡喃糖基 2 5 (R) 呋甾 Δ5,2 0 ( 2 2 ) 二烯 3β,2 6 二羟基 3 O [α L 鼠李吡喃糖基 (1→ 2 ) ] β D 葡吡喃糖苷 (III)。 结论 I为新化合物 ,命名为黄山药皂苷C ,II,III为首次从本属植物中分得。
AIM To investigate the chemical constituents of the rhizomes of Dioscorea panthaica Prain et Burkill and look for new active compounds. METHODS The compounds were isolated with silica gel and HPLC, and their structures were elucidated on the basis of chemical evidences and spectral analysis (IR, ESI MS, 1HNMR , 13 CNMR , DEPT, 1H 1H COSY, HMQC, HMBC, NOESY). RESULTS Three steroidal saponins were isolated from the rhizomes of Dioscorea panthaica . They were elucidated as 26 O β D glucopyranosyl 3β,26 diol 23( S ) methoxy 25( R ) Δ 5,20(22) diene furosta 3 O [α L rhamnopyranosyl (1→2) O α L rhamnopyranosyl (1→4)] β D glucopyranoside (I), pseudoprotodioscin (II), 26 O β D glucopyranosyl 3β,26 diol 25( R ) Δ 5,20(22) diene furosta 3 O α L rhamnopyranosyl (1→2) O β D glucopyranoside (III). CONCLUSION Compound I is a new steroidal saponin and was named as dioscoreside C. Compound II and III were obtained from this genus for the first time.
出处
《药学学报》
CAS
CSCD
北大核心
2001年第1期42-45,共4页
Acta Pharmaceutica Sinica
基金
国家中医药管理局资助!项目 (95 80 17)
卫生部资助!项目 (96 1 2 31)
关键词
黄山药
甾体皂苷
黄山药皂苷C
分离
鉴定
Dioscorea panthaica Prain et Burkill
steroidal saponins
dioscoreside C
