摘要
目的 探讨肾移植稳定病人血清sE 选择素 (sCD6 2E)浓度与其淋巴细胞表面配体L 选择素 (CD6 2L)表达的关系和意义。方法 利用单克隆抗体 流式细胞仪荧光免疫技术和双抗体夹心酶联免疫法 ,测定 10例移植稳定病人术后不同时间CD6 2L表达及sCD6 2E浓度。结果 术后CD6 2L表达率由 (31.5± 14 .5 ) %渐升至 (5 3 .8± 15 .7) % ,差异有非常显著性 (P <0 .0 1) ,15d时明显高于术前和正常对照组 (P <0 .0 5 ) ;术后sCD6 2E浓度则由 (31.7± 10 .0 ) μg/L渐降至 (11.9±2 .5 3) μg/L (P <0 .0 1) ,9d时明显低于术前组 (P <0 .0 5 )。sCD6 2E和CD6 2L之间呈负相关 (r =-0 .8183 ,P <0 .0 5 )。结论 血清sCD6 2E浓度降低与淋巴细胞CD6 2L表达增加相关。术后监测sE 选择素浓度和CD6
Objective To investigate the correlation between the soluble E selectin (sCD62E) in sera and expression of CD62L on the surface of lymphocyte from peripheral blood of renal allograft recipients in stable course and its implication. Methods The dynamic changes of CD62L levels on lymphocyte and the sCD62E were measured by using mAb FACS immunofluerescence technique and sandwich ELISA correspondingly in 10 renal allograft recipients in stable course after operation. Results The levels of CD62L on lymphocyte were increased gradually from (31.5±14.5) % to (53.8±15.7) % ( P <0.01)after operation, and significantly higher than that in the normal control group (44.0±14.3) % ( P <0.05) and the pre operation group (38.5±16.8)% ( P <0.05) at 15th day. The sCD62E was decreased from (31.7±10.0) μg/L to (11.9±2.53) μg/L ( P <0.01) and reached a stable level of (12.4±2.87) μg/L at 9th day after operation, and significantly lower than that of the pre operation group (37.4±27.5) μg/L ( P <0.05). A negative correlation was found between the two parameters ( r =-0.818 3, P <0.05). Conclusion The decrease of sCD62E concentration in sera are correlated with the upregulation of CD62L expression on lymphocyte to some extent in stable course after renal allograft transplantation. The dynamic investigation of sE selectin in sera and CD62L on lymphocyte may be helpful for valuation of the immune statue of the recipients after kidney transplantation. [
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2001年第1期33-34,共2页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助! (39570 2 0 0 )
