摘要
利用5种方法对黑曲霉AS3.350进行诱变,研究不同诱变方法对黑曲霉产木聚糖酶的影响,获得高产木聚糖酶的突变株。结果表明,紫外诱变获得的最优突变株酶活是出发菌株的2.18倍,诱变效果最为明显,其次是快中子辐照诱变,获得的最优突变株酶活是出发菌株的1.72倍,硫酸二乙酯诱变、亚硝酸钠诱变、亚硝基胍诱变获得的最优突变株酶活分别是出发菌株的1.51倍、1.46倍、1.38倍,物理诱变比化学诱变对黑曲霉产木聚糖酶更为敏感。
Based on the Aspergillus niger AS3.350, the mutants were brewed through inducing five treatments.The effect of different mutation methods on enzyme production ability of Xylanase was disscused and obtained mutant strain of xylanase production high ability. The results indicate that, the mutagenesis of UV irradiation was most obvious, and the xylanase activity was 2.18 times of the original strain.Mutagenic effect of the fast neutron irradiation mutagenesis was second, the xylanase activity was 1.72 times of the original strain.After mutagenesis by diethyl sulfatemutagenesis, sodium nitrite, nitrosoguanidine, xylanase activity of the best mutant strains were 1.51 times, 1.46 times, 1.38 times.Physical mutation is more sensitive than chemical mutagenesis on increasing xylanase activity by Aspergillus niger .
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第12期146-150,共5页
Biotechnology Bulletin
关键词
紫外诱变
快中子辐照
亚硝基胍诱变
硫酸二乙酯诱变
亚硝酸钠诱变
Uvirradiation Fast neutron irradiation Nitrosoguanidine mutation Diethyl sulfate mutagenesis Sodium nitrite induced
