摘要
目的:探讨HER2、VEGF的表达和MVD水平及这三者与胃癌的临床病理和生物学特性的关系。方法:胃癌患者26例,其中14例患者接受了全胃切除术,12例患者行胃癌活检,在癌组织中心获得有效标本设为胃癌组,以距离癌组织中心5 cm的正常癌组织为正常组。RT-PCR法检测两组胃组织中HER2 mRNA的表达;免疫组织化学检测胃组织中VEGF的表达;计算各组微血管密度。结果:与正常组相比,胃癌组HER2 mRNA表达明显增加;正常组胃组织VEGF平均灰度值为21.51±4.64,而胃癌组胃组织VEGF平均灰度值为167.23±18.85,两组相比有明显差异;正常组胃组织MVD为13.44±5.34个,与正常组相比,胃癌组胃组织MVD水平(65.74±9.87个)明显增高;HER2与患者TNM分期成正相关,VEGF与患者性别及TNM分期均成正相关,而MVD与患者临床病理特征无相关性。结论:血管内皮生长因子的表达及与胃癌患者临床病理特征的相关性表明,HER2、VEGF两个分子生物标志物在肿瘤的发生发展中具有重要作用,其能够被用来作为预测的侵袭性胃癌预后的重要参数。
Objective： To investigate HER2, VEGF expression and MVD in gastric and the relationships between them and clinical pathology and biological characteristics. Methods： 26 patients with gastric cancer, 14 patients underwent total gastrectomy, 12 patients with gastric biopsy, cancer center at a distance of 5 cm for the normal group, norm. RT-PCR Were detected to HER2 mRNA expression in gastric tissue; immunohistochemical was used to detect VEGF expression in gastric tissue; microvessel density calculated for each group. Results： Compared with the normal group, gastric cancer HER2 mRNA expression was significantly increased; normal gastric tissue VEGF average gray value of 21.51 ± 4.64, and gastric cancer gastric tissue VEGF average gray value of 167.23 ± 18.85, compared the two groups significantly difference; normal gastric tissue MVD was 13.44 ± 5.34, compared with the normal group, MVD in gastric cancer group level （65.74 ± 9.87 unit） was significantly higher; HER2 patients with TNM stage and positive correlation, VEGF and patient gender and TNM stage were positively correlated with the clinical and pathological features. Conclusions： Vascular endothelial growth factor expression and clinicopathological features of gastric cancer patients showed correlation show HER2 and VEGF has an important role in tumor development, which can be used as predictor＇s parameters ofinvasive cancer.
出处
《现代生物医学进展》
CAS
2013年第33期6544-6547,6561,共5页
Progress in Modern Biomedicine
基金
海南省自然科学基金项目(813193)
