摘要
目的：研究有氧运动干预对大鼠创面愈合的影响及免疫炎症方面的可能机制,为运动在创面愈合中的应用提供参考.方法：SPF级8周龄健康SD大鼠40只随机分为健康安静组（A组）、创伤安静组（B组）、伤后运动组（C组）、伤前长期运动及伤后运动组（D组）,雌雄各半.切除背部皮肤进行创伤造模.D组伤前有氧运动锻炼4周,C、D组伤后给予有氧运动干预.伤后第3、10、17天断尾取血备血清,ELISA法测血清IL-1β、TNF-α、TGF-β1,同时观察创面愈合情况.结果：两种方式的运动干预使皮肤创面愈合加快2～3d;伤后大鼠血清TNF-a和IL-1β含量明显上升（P＜0.05）,C、D组低于B组（P＜0.05）,并随愈合进展逐步下调（P＜0.05）,而C、D组血清TGF-β1整体低于B组（P＜0.05）.结论：有氧运动可能通过改善创伤机体免疫炎症反应及多个细胞因子的平衡来促进创面愈合.
Objective:To study the effects of aerobic exercise intervention on rats'wound healing and its'possible mechanism of immune inflammation,and give reference on applying exercise intervention in wound healing.Methods:40SPF grade and 8weeks old SD rats were randomly divided into the control group(group A),the wound model group(group B),the exercise after wound group(group C),the exercise both before and after wound group(group D),and half by half in gender.The dorsal skin was ablated and made as trauma model.Group D did aerobic exercise for 4weeks before wound.Both group C and D did aerobic exercise after wound.Blood of rats in all groups were collected by cutting tails on 3rd,10th,17th day during wound healing for preparing serum.IL-1β、TNF-α、TGF-β1of rats'serum were tested by ELISA while wound healing conditions were observed.Results:Two ways of exercise intervention on the skin wound healing resulted in 2~3days acceleration;after the injury the TNF-a and IL-1βin rat serum increased obviously(P<0.05),but TNF-a and IL-1βof C,D groups were lower than those of B group(P<0.05)with the healing progressing gradually while TGFβ1in serum of C,D was overall lower than group B(P<0.05).Conclusion:Aerobic exercise may improve trauma immune inflammatory reaction and multiple cytokine balance to promote wound healing.
基金
广东省自然科学基金项目(S2011010003089)
