摘要
建立了利咽颗粒中蝙蝠葛碱、蝙蝠葛苏林碱的含量测定方法。采用DiamonsilTMC18色谱柱(250 mm×4.6 mm,5μm),以乙腈-体积分数为0.05%三乙胺水溶液为流动相,流速为1.5 mL·min-1,检测波长为284 nm,进样量20μL,柱温为35℃。在上述色谱条件下,蝙蝠葛碱和蝙蝠葛苏林碱在1.106～8.848μg·mL-1(r=0.9995)、5.6～28μg·mL-1(r=0.999 8)范围内线性关系良好,蝙蝠葛碱和蝙蝠葛苏林碱的平均加样回收率(n=9)均为98.7%;RSD分别为1.6%、1.5%。该法简便、准确、重复性好,可用于利咽颗粒中蝙蝠葛碱和蝙蝠葛林碱的含量测定。提高了利咽颗粒的质量控制标准,为其临床用药的安全性和有效性提供了质量保证。
A HPLC method was established to determine contents of dauricine and daurisoline in Liyan Granules. Sample were analyzed on an DiamonsilTMC18（250 mm×4.6, 5 μm）column eluted with acetonitrile and water containing 0.05% triethy lamine as mobile phase in a gradient model; the flow rate was 1.5mL·min-1, the detective wavelength was set at 284 nm,and the column temperature was set at 35℃. The linear response ranges were 1.106~8.848μg·mL-1 （r=0.999 5） and 5.6~28μg·mL-1 （r=0.999 8） for dauricine and daurisoline ,respectively,The average recoveries （n=9） of these compounds all were 98.7%,and the RSDs were 1.8%and 1.5%, respectively. The method is simple,accurate and reproducible for determination of dauricine and dauri-soline in Liyan Granules simutaneously, and its quality standard can be improved, the method can provide a quality assurance for medicinal safety and clinical effectiveness of Liyan granules.
出处
《当代化工》
CAS
2013年第11期1528-1531,共4页
Contemporary Chemical Industry
