摘要
从29株红曲霉菌(Monascus sp.)中筛选得到的M2为出发菌株,进行UV照射、LiCl诱变以及硫酸二乙酯处理与LiCl复合诱变三次诱变,筛选获得Monacolin K的产量显著提高的菌株MS-12。对液态发酵培养基、发酵温度、接种量以及摇床转速等因素进行优化。在最优条件下MS-12液态发酵Monacolin K的产量显著提高,达到264.7μg/mL,桔霉素含量仅为0.026ng/mL。
From 29 strains of Monascus sp. ,one strain（M2） which produced Monacolin K higher than others was chosen to be original strain. The strain then treated with UV,LiCI,ethyl sulfate and LiCI,a mutant strain of Monascus sp. named MS-12 was obtained which could produce much higher Monacolin K than M2. These optimizations of the liquid-state fermentation medium,fermentation temperature,inoculum concentration and the shaking speed were done. Under optimal conditions,the Monacolin K content of MS-12 improved significantly, reached 264.7ug/mL. And the citrinin content was only 0.026ng/mL.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第24期296-301,共6页
Science and Technology of Food Industry
基金
山东省优秀中青年科学家科研奖励基金计划(06BSC07016)
