摘要
目的:优选鸡骨草中总三萜酸的半仿生法提取工艺。方法:采用UV测定总三萜酸含量。以总三萜酸提取率为指标,在单因素试验基础上,通过正交试验考察提取温度、酸提取时间、碱提取时间、料液比对鸡骨草总三萜酸提取工艺的影响,并与传统水提法、超声波法进行比较。结果:最佳提取工艺条件为料液比1∶25,恒温水浴温度100℃,酸(pH 2)提取时间55 min,碱(pH 8)提取时间25 min;总三萜酸提取率达0.123%,高于传统水提法(0.089%),但低于超声波法(0.304%)。结论:采用半仿生法提取鸡骨草中总三萜酸的工艺具有绿色环保、成本低等优点。
Objective： To optimize semi-bionic extraction technology of total triterpene acids from Abri Herba. Method： The content of total triterpene acids was determined by UV.With yield of total triterpene acids as index,based on single factor tests,orthogonal test was adopted to optimize semi-bionic extraction technology of total triterpene acids by taking extraction temperature,ratio of solid-liquid,acid and alkali extraction time as factors,then compared with water extraction method and lower than ultrasonic method. Result： Optimum semi-bionic extraction technology was as following：extraction temperature at 100℃,solid-liquid ratio of 1：15,acid（pH 2） extraction time 55 min,alkali（pH 8） extraction time 25 min;Under these conditions,yield of total triterpene acids was 0.123%,which was higher than water extraction method（0.089%） and lower than ultrasonic method（0.304%）. Conclusion： Optimized semi-bionic extraction process had advantages of green environmental protection,low cost and so on.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第24期46-48,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西教育厅科研立项项目(201106LX584)
关键词
总三萜酸
鸡骨草
半仿生法
提取工艺
正交试验
单因素试验
total triterpene acids
Abri Herba
semi-bionic
extraction technology
orthogonal test
single factor test
