摘要
【目的】探讨柑橘属五大基本类型不同组织(花、果皮和叶片)中类黄酮组成与含量差异,为合理开发利用柑橘资源提供科学依据。【方法】采用超高效液相色谱二极管阵列紫外-可见光检测器(UPLC-PDA)测定柑橘属五大基本类型10个典型代表种的花、果皮和叶中11种类黄酮含量。【结果】所分析的五大基本类型中,大翼橙类和柚类主要含柚皮苷,枸橼类、橙类和宽皮柑橘类主要以橙皮苷为主,其中广西沙田柚花中柚皮苷含量最高(11 987.1 mg·kg-1FW),鹅蛋红橘果皮中橙皮苷含量最高(14 794.4 mg·kg-1FW);代表类型各组织中类黄酮含量差异显著(P<0.05),花、果皮和叶片中11种类黄酮总含量最高的分别为广西沙田柚(12 198.5 mg·kg-1FW)、鹅蛋红橘(16 744.1 mg·kg-1FW)和台湾椪柑(8 879.2 mg·kg-1FW);不同类型柑橘各组织中的类黄酮含量呈现不同变化模式,同一类型不同组织间只有丰度差异,大翼橙类中花>叶>果皮,枸橼类中花>果皮>叶,橙类中果皮>花>叶,柚类中花>果皮>叶,宽皮柑橘类中果皮>叶>花。【结论】中国特有柑橘属植物含有丰富的类黄酮,不同柑橘基本类型及其组织间类黄酮变化很大,应针对各自不同特点加以科学利用。
[ Objective ] The aim of this study was to evaluate the flavonoid composition and content in flower, peel and leaf of the basic genotypes of genus citrus in an attempt to provide information for the utilization of resource.[Method]Eleven flavonoids in different organs （flower, peel, leaf） of 10 typical genotypes representing five basic types of the genus citrus were determined by UPLC-PDA. [Result] Naringin is the major flavonoid in Papeda and Cephalocitrus, and hesperidin is the major flavonoid in Citrophorum, Aurantium and Sinocitrus. The highest naringin content was detected in the flowers of Guangxishatianyou （11 987.1 mg＇kg＇lFW）, whlie the highest hesperidin content was detected in the peel of Edanhongju （14 794. mg＇kglFW）. Significant differences were observed in falvoniod contents of different Citrus plant organs. The highest total contents of 11 flavonoids of flower, peel and leaf were Guangxishatianyou （12 198.5 mg.kg-lFW）, Edanhongju （16 744.1 mg＇kglFW） and Taiwanpenggan （13 396.3 mg.kglFW）, respectively. The flavonoids content of flowers, peel, leaves in different genus citrus showed different variation patterns, However, there was only an abundance difference among different organizations of the same genus citrus, the orders of total flavonoids content variation in Papeda, Citrophornm, Aurantium, Cephalocitrus, Sinocitrus were flower〉leaf 〉peel, flower〉peel 〉leaf, peel〉 flower〉leaf, flower〉leaf〉peel, peel〉leaf〉flower, respectively. [Conclusion] The citrus genotypes native to chinaare rich in flavonoids, and obvious variations were observed between different types and tissues. The utilization of citrus resources should be on the basis of their respective characteristics.
出处
《中国农业科学》
CAS
CSCD
北大核心
2013年第22期4753-4762,共10页
Scientia Agricultura Sinica
基金
国家自然科学基金项目(31171930)
中央高校基本科研业务费专项(XDJK2013A014)
现代农业(柑橘)产业技术体系建设专项(CARS-27)
引进国际先进农业科学技术"948"计划项目(2012-Z13)
重庆市"创新团队建设计划"项目(KJTD201333)
关键词
柑橘
柑橘5大基本类型
组织
类黄酮
citrus
five basic types oft_he genus citrus
tissues
flavonoid
