摘要
目的 探讨幽门螺杆菌 (Hp)对甲硝唑的耐药机制。方法 (1)甲硝唑选择压力试验 ;(2 )SDS PAGE电泳 ;(3)硝酸盐还原试验 ;(4 )革兰阴性菌 95种底物相关酶类检测。结果 Hp由敏感株突变为耐药株后 ,1 甲基琥珀酸、琥珀酸、D 丙氨酸相关酶类活性降低 ;L 岩藻糖、6 磷酸葡萄糖相关酶类活性增高。
Objective To probe the mechanism of metronidazole resistance to Helicobacter pylori (Hp). Methods (1) The selective pressure test of metronidazole. (2) SDSPAGE electrophoresis. (3) Nitrate reduction test. (4) Test for detecting the enzyme activities associated with 95 substrates of gram negative. Results After the mutation of Hp from sensitive to resistant strains, the activities of enzymes associated with monomethyl succinate, succinic acid and Dalanine is decreased, and those associated with Lfucose 6phosphate glucose is increased. Conclusions The metronidazole resistance of Hp is associated with the metabolism and the change of enzyme activities.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2000年第6期368-370,共3页
Chinese Journal of Digestion
关键词
幽门螺杆菌
甲硝唑
耐药机制
Helicobacter pylori
Metronidazole
Antibiotic resistance
