摘要
为了明确104份苦荞种质之间的遗传差异，利用SSR分子标记对其遗传多样性进行了研究。15对SSR引物共扩增出86个清晰条带，其中多态性条带56个，占总数的65．1％。104份材料之间的遗传相似系数变幅为0．83～1．00；在相似系数为0．88时，104份材料可分成5组。西北、西南地区省份的材料存在着丰富的遗传多样性，而内蒙古赤峰地区的37份苦荞则划分在同一个组中，表明赤峰地区的苦荞资源遗传基础较狭窄。由于一些地方材料可能存在同名异物、同物异名的情况，因此，对苦荞资源进行分子水平遗传多样性评价很有必要。
To make clear the genetic differences of 104 tartary buckwheat accessions, SSR molecular markers were used to study the genetic diversity. Fifteen pairs of SSR primers were used and eighty-six clear bands were amplified, in which 56 were polymorphic, accounting for 65.1%. The diversity coefficient of 104 accessions ranged from 0.83 to 1.00. At coefficient 0.88 ,the 104 tartary buckwheat accessions could be clustered into 5 groups. Accessions from northwest and southwest of China are of high genetic diversity, while the genetic base of those from Chifeng in Inner Mongolia is relatively narrow. Some the same accessions may have different names, and some different accessions may have the same names, so it is necessary, especially for some rural accessions, to evaluate the genetic diversity with molecular markers.
出处
《作物杂志》
CAS
CSCD
北大核心
2013年第6期13-18,共6页
Crops
基金
内蒙古自治区科技计划项目(20110707)
赤峰市农牧科学研究院基金项目(201101)
关键词
苦荞
遗传多样性
SSR
聚类分析
Tartary buckwheat
Genetic diversity
SSR
Cluster analysis 1R
