摘要
目的:对长期居住在天津市某镇电子垃圾处理较集中的3个村庄的居民进行细胞遗传学研究,分析电子垃圾污染物对人体的遗传损伤效应。方法:在电子垃圾处理区居民中随机选择171位村民作为暴露组,进行染色体畸变(CA)和胞浆阻滞微核(CBMN)分析,并进行单细胞凝胶电泳检测DNA损伤。选取与该镇毗邻区域且从未接触电子垃圾处理的30位村民(男女各半)作为对照组。暴露组按照性别和年龄分组,分别观察不同性别和年龄组的CA、CBMN和DNA损伤水平。结果:暴露组染色体总畸变率为5.50%,与对照组(1.70%)差别有统计学意义(P<0.01);暴露组微核率为16.99‰,对照组为3.47‰,差异有统计学意义(P<0.01);单细胞凝胶电泳检测显示暴露组尾部DNA百分含量(TDNA,%)、尾矩(TM)、Olive尾矩(OTM)较对照组均显著升高(P<0.01)。染色体畸变率、微核率和DNA损伤水平女性均明显高于男性(P<0.01),而在各不同年龄组间未见明显差异(P>0.05)。结论:电子垃圾污染物是潜在的遗传诱变剂,能够造成污染地区人群的细胞遗传学损伤,不能忽视其对当代及其子代健康的有害影响。
OBJECTIVE：To study cytogenetics change on the residents of three villages in electronic wastes recycling area of a town in Tianjin,and analyze the damaging effect to human beings caused by pollutants of electronic wastes. METHODS：171 residents were randomly selected as population sample chromosome aberration （CA）, cytokinesis-blockmicronucleus （CBMN） test and single cell gel electrophoresis （SCGE） were performed. 30 residents who lived near the electronic waste recycling town but never had contact with electronic wastes were recruited as control group. To assess CA,CBMN and DNA damage of samples in different gender and age,exposure group was divided into subgroups according to gender and age. RESULTS：Total rate of CA of the 171 residents was 5.50%,and CBMN rate was 16.99‰. Significant differences were found in both. The same difference was found in DNA percent in the tail （TDNA,%）,tail moment （TM） and Olive tail moment （OTM） detected by SCGE compared with control group. The level of chromosome aberration,micronucleus rate and DNA damage of female group were significantly higher than that of male group,but no significant difference was found among three age groups. CONCLUSION：The pollutants of electronic wastes were latent genetic mutagens indeed,causing cytogenetics damage to the population who have been exposed. The harmful effect to humans and their offsprings should not be ignored.
出处
《癌变·畸变·突变》
CAS
CSCD
2013年第6期448-453,共6页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金(31170804
31240052)
天津市科技支撑重点项目(12ZCZDSY03400
13JCYBJC23500
12JCYBJC15300)
