摘要
为寻找人参病原真菌的生防菌株,采用传统的分离纯化方法,从人参新鲜根中分离内生真菌,并通过平板对峙法筛选高效广谱的菌株;利用形态学与18SrDNA序列分析相结合的手段对菌株进行鉴定.结果表明:从3年生人参新鲜根中分离得到86株内生真菌,其中,Ns-3、Ns-16及Ns-38菌株对人参7种病原菌均有高效广谱抑菌作用,Ns-3对人参根腐病菌的抑菌率达到63.33%,Ns-16对人参灰霉病菌的抑菌率达到69.63%,Ns-38对人参锈腐病菌的抑菌率达到62.34%.通过形态学与18SrDNA序列分析,Ns-3、Ns-38为半知菌亚门青霉属真菌(EpicoccumSP.),Ns-16为半知菌亚门附球菌属黑附球菌(Epicoccum nigrum).为人参内生生防制剂的开发和利用提供了依据,也为开发经济环保的生物农药提供了菌种资源.
In order to find biocontrol strains of seven pathogens of Panax ginseng, endophytic fungi were isolated from fresh root of Panax ginseng by traditional methods of separation and purification. The high-efficiency and wide-spectrum antagonistic endophytes were screened using dual culture on potato dextrose agar. The strains were identified by a method which performed by the morphological and 18S rDNA sequence analysis. It was showed that 86 strains endophytic fungi were isolated from 3- year-old fresh root of Panax ginseng. Three of them named Ns-3,Ns-16 and Ns-38 are highly effective broad spectrum antimicrobial effect to seven kinds of pathogenic strains of Panax ginseng. The percentage of bacteriostasis of Ns-3 was reached 63.3% to Fusariurn solani of Panax ginseng. The percentage of bacteriostasis of Ns-16 was reached 69.63% to Botrytis cinerea of Panax ginseng. The percentage of bacteriostasis of Ns-38 was reached 62.34% to Cylindrocarpon destructans of Panax ginseng. The results of morphological and 18S ribosomal DNA sequence analysis showed that Ns-3, Ns-38 were Epicoccum SP.. Ns-16 were Epicoccurn nigrum. The study provide the basis and strains resources, for which development and use of endophytic biocontrol agents of Panax ginseng and economic and environmentally friendly bio-pesticides.
出处
《东北师大学报（自然科学版）》
CAS
CSCD
北大核心
2013年第4期107-113,共7页
Journal of Northeast Normal University(Natural Science Edition)
基金
国家科技支撑计划重大项目(2011ABI03B0102)
国家自然科学基金资助项目(31270371)
关键词
人参
内生真菌
拮抗
病原菌
Panax ginseng
endophytic fungi
antagonistic bacteria
