摘要
目的:测量糖尿病性视网膜病变(DR)行玻璃体切除术(PPV)患者玻璃体样品中白细胞介素-8(IL-8),肿瘤坏死因子-α(TNF-α)的水平,与对照组比较结果并探讨其对DR的影响。方法:将伊斯坦布尔Bilim大学眼科系的57例(57眼)DR患者和22例黄斑裂孔未伴有增生性玻璃体视网膜病变患者分为研究组和对照组。对所有79例患者行3切口,20G PPV。PPV手术眼内灌注前,用Vitrector抽吸0.5mL玻璃体样品并稀释。样品转至冷藏室,存放在-70℃。IL-8和TNF-α的测量结果用酶联免疫吸附法以pg/mL为单位计算。结果:DR患者玻璃体样本中IL-8水平[82.7891±74.08700(0.08-307.09)pg/mL]明显高于对照组患者[2.9805±3.77546(0.08-18.53)pg/mL](P<0.001)。同样,DR患者TNF-α水平[18.0007±13.90015(2.32-51.11)pg/mL]也显着高于对照组[1.7005±1.26949(0.1-5.17)pg/mL](P<0.001)。结论:在视网膜新生血管形成中起重要作用的TNF-α和作为炎症和血管生成介体的IL-8水平在DR患者玻璃体样本中明显高。
AIM：To measure the interleukin-8 （ IL-8 ） and tumor necrosis factor-α（ TNF-α） levels in vitreous samples obtained the patients undergoing pars plana vitrectomy （ PPV） due to diabetic retinopathy （ DR） , then to compare these results with those of the control group and to state their impact on DR pathogenesis.
METHODS：From Istanbul Bilim University Ophthalmology Department, 57 eyes of 57 diabetes mellitus （ DM ） patients who ha d been diagnosed with proliferative diabetic retinopat hy and 22 cases of macular hole,w ith no pro liferative vitreoret inopathy were included in the study as the study and the control groups resp ectively.All of the 79 patie nts underwent a 3- port, 20 gauge PPV. Vitreous sampleso f 0.5mL were aspirated with vitrector at the beginning of the PPV operation before the intraocular infusion and being dilute d.Samples were transferred to the freezer to be stored at -70℃.Re sults of IL-8 and TNF-αwere calculated as pg/mL with ELISA method.
RESULTS： IL-8 levels [ 82.7891 ±74.08700 （ 0.08-307.90 ） pg/mL ] in which vitreous samples obtained DR patients during vitrectomy were is gnifican tly elevated when compar ed to IL-8 levels [2.9805 ±3.77546 （0.08-18.53） pg/mL] of control patients （P〈0.001）.Similarly, TN F-αlevel [18.0007±13.90015（2.32-5 1.11）pg/mL] was al so significa ntly elevated in DR patients when compared to control patient s＇TNF-αlevel [ 1.07 05 ±1.296 49 （ 0.1-5.17）pg/mL] （P〈0.001）.
CONCLUSION：The levels of TNF-α, which plays a role in retinal neovascularization, and, IL-8, which acts as an i nflam matory and angiogenic mediator were fou nd to be high in DR patients.
出处
《国际眼科杂志》
CAS
2013年第12期2365-2369,共5页
International Eye Science
