摘要
为揭示复合菌剂及平衡施肥对烤烟抗逆、健株和提质增效的作用,采用田间试验研究了复合菌剂与有机无机肥配施对烤烟生长发育和不同生育期烟株根系、叶片保护酶活性以及产量品质的影响。结果表明,复合菌剂与有机无机肥配施能显著改善烤烟农艺性状,提高烟株根系和叶片超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)和苯丙氨酸解氨酶(PAL)等保护酶活性,降低黑胫病、赤星病、花叶病等的发病率和病情指数。相关分析表明,烤烟根系PAL,SOD,POD与叶片的PAL,SOD,POD等保护酶活性呈正相关。复合菌剂与有机无机肥配施还提高了烟叶还原糖和总糖含量,尤其是烟叶钾含量显著增加,烟叶化学成分更趋协调。复合菌剂结合75%有机肥与无机肥配施比例有利于烤烟生长发育,提高其抗逆性、产量和品质。
In order to investigate the co-effects of compound microbial inoculum and organic-inorganic fertilizer on the resistance, growth, yield and quality of flue-cured tobacco, the activity of various defensive enzymes in tobacco roots and leaves at different growing stages as well as the changes of tobacco yield and quality were determined with field trials. The results indicated that proportionate application of compound microbial inoculum with organic and inorganic fertilizers significantly improved the agronomic character of tobacco, enhanced the activities of defensive enzymes, such as superoxide dismutase （SOD）, catalase （CAT）, peroxidase （POD）, and phenylalanine ammonia lyase （PAL） in tobacco roots and leaves, and reduced the morbidity and disease index relative to tobacco black shank disease, tobacco mosaic virus disease and tobacco brown spot disease. Correlation analysis showed that the activities of defensive enzymes （PAL, SOD, and POD） in tobacco roots positively correlated to those in tobacco leaves. Proportionate application increased the contents of total sugar and reducing sugar in tobacco leaves, especially increased potassium content significantly, which made the chemical composition in tobacco leaves more harmonious. The experiments indicated that the application of compound microbial inoculum together with 75% organic and inorganic fertilizers was beneficial to the growth, development of flue-cured tobacco and increasing its yield, quality and resistance to stress.
出处
《烟草科技》
EI
CAS
北大核心
2013年第12期67-73,共7页
Tobacco Science & Technology
基金
四川省烟草专卖局科技项目“肥药双效烟草专用生物有机肥研究与开发”(201101006)和“四川省植烟土壤高效功能微生物分离与筛选”(201202006)
关键词
烤烟
复合菌剂
有机肥
无机肥
保护酶
生长发育
Flue-cured tobacco
Compound microbial inoculum
Organic fertilizer
Inorganic fertilizer
Defensive enzyme
Growth and development
