摘要
从酒曲中筛选出一株高产蛋白酶的菌株,命名为OPY6,初步鉴定为假单胞茵Pseudomonas sp.,经P1ackett-Burman Design设计和Box-Behnken响应面设计对其产酶培养基做了优化,最优产酶条件为:葡萄糖2.83%,淀粉0.87%,酵母膏0.55%,氯化钙0.001%,氯化钠0.5%,黄豆粉1%,初始pH=7.0,在最佳培养基添加量下蛋白酶活提高到134.718 U/mL;又对该酶在水产蛋白降解过程中的酶解效果进行了评价。
A strain with high protease activity was screened out from the wine cube and was named OPY6. It was prelimi- narily identified as Pseudomonas sp. The optimum culture medium and conditions for protease production of OPY6 were in- vestigated by Plackett-Burman Design and Box-Behnken response surface. The results showed that the optimum conditions for enzyme production were： glucose 2.38% , starch 0.87% , yeast extract 0.55% , CaC12 0. 001% , NaC1 0.5% , soybean meal 1%, initial pH = 7.0, while the activity of protease had increased to 134.718 U/mL. Besides, the hydroly- zation effect in marine protein by the protease was evaluated.
出处
《工业微生物》
CAS
CSCD
2013年第6期26-32,共7页
Industrial Microbiology
基金
中央高校基本科研业务费专项资金资助(201262021)
关键词
假单胞菌
中性蛋白酶
响应面法
培养基优化
酶解效果
Pseudomonas sp.
neutral protease
response surface method
medium optimization
enzymolysis effect
