摘要
建立五味子中五味子醇甲、五味子甲素、五味子乙素、五味子丙素4种成分的高效液相色谱测定方法,分析不同样地间气候因子和海拔高度对五味子木脂素类含量的影响,确定吉林省五味子最适栽培地区的气候特点。采用高效液相色谱法,Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇(A)-水(B)梯度洗脱,检测波长254 nm,柱温35℃,流速1 mL·min-1,该测定方法重复性、稳定性和精密度均良好。并用SPSS软件分析气候因子和木脂素相关性,结果发现采集于吉林省集安市、柳河县、安图县和抚松县的五味子成熟果实中五味子醇甲质量分数均高于0.4%,达到质量要求,但是,五味子醇甲、五味子甲素和五味子乙素含量与样地海拔高度间呈明显的线性负相关关系,即随海拔高度增加呈下降趋势,特别是五味子醇甲的负线性拟合系数达到0.844 1,具有显著性,而五味子丙素与样地海拔高度的相关性不明显。与五味子木脂素中五味子醇甲、五味子乙素以及4种木脂素含量呈显著正相关的气候因子主要是温度因子(年均气温、7月最高温、7月平均温、1月最低温、1月平均温)和降水因子(年均降水量),说明较高的温度和降水量有助于五味子中木脂素的形成和累积。建议五味子栽培区域应尽可能选择在温暖多雨的低海拔地区。
The contents of schisandrin, schizandrin A, B and C were determined by HPLC, and the effects of the climate factors and altitude on lignin contents were analyzed in order to select the optimal cultivation area of S. chinensis. The lignin contents were analyzed by HPLC using a ZORBAX SB-CIs column （4.6 mm×250 mm, 5μm）. The column temperature and detection wave length were set at 35 ℃ and 254 nm, respectively. Methanol-water was used as the mobile phase in gradient elution mode and the flow-rate was 1.0 mL min- 1. The method had a good repeatability, stability and accuracy. The correlation of climate factors and lignins contents was analyzed by SPSS software. The results showed that the schizandrin A content in S. chinensis fruits were higher than 0.4% in Ji＇an, Liuhe, Antu and Fusong in Jilin province, which met the quality requirement. It had significant linear negative correlation relationship between sehisandrin, sehizandrin A, B and altitude, the contents decreased with the increase of altitude. The significant negative linear fitting coefficient was 0. 844 1 between schisandrin and altitude ; but it had not significant correlation between sehizandrin C and altitude. A significant positive correlation of climate factors and the contents of S. chinensis lignins were mainly the temperature factors （the average annual temperature, the highest temperature in July, the average temperature in July, the highest temperature in January, the average temperature in January）and precipitation factor（ average annual precipitation）, which reveals that higher tempera- ture and precipitation were helpful to the formation and accumulation of lignins of S. chinensis. So the cultivation area of S. chinensis should be in the low elevations region with warm and rainy climate.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第24期4281-4286,共6页
China Journal of Chinese Materia Medica
基金
吉林省医药产业发展重大专项(YYZX201007)
科技成果转化计划项目(20130305049YY)
关键词
五味子
木脂素
高效液相色谱
含量测定
气候因子
相关性分析
最适栽培区域
Schisandra chinensis
lignin
high performance liquid chromatography
content determination
climate factors
correlation analysis
optimal cultivation area
