摘要
以亚硝基胍、紫外线和丝裂霉素处理栖热杆菌FD3009(Thermus Sp.FD3009),从诱交群体中选得抗紫外线、丝裂霉素和氟尿嘧啶的菌株FD 20·1(Ther-mus Sp.FD20·1).FD20·l对高温和pH的适应范围与FD3009一样,但菌落的色泽较浅,呈淡黄色,其耐热DNA聚合酶的比活力比FD 3009提高了37.8%。
Thermus sp. FD3009 was treated with nitrosoquanidine, mitomycin C, and UV
light and a resistant mutant strain FD20·1 was obtained. FD20·1 was not different
from FD3009 with respect to the temperature and pH range of growth, but differed
in being paler in colour action. The DNA polymerase production of FD20·1 was
38.7% higher than that of FD3009. FD20·1 could easily be induced to show high
resistance toward mitomycin C, but the resistance declined gradually on subcultu-
ring. The high resistance toward mitomycin C seems also to be related to high produc-
tivity of DNA Polymerase.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1991年第4期399-404,共6页
Journal of Fudan University：Natural Science
关键词
栖热杆菌
DNA
聚合酶
诱变
育种
thermophilic bacteria, polymerization enzyme, mutation breeding, mutagens
DNA polymerzation enzyme, nitrosoquanidine, UV light, mitomycin.
