摘要
从东太平洋热液区E53站位的深海沉积物样品中分离出1株能在65℃生长的嗜热菌(DYth03).该菌的16S rDNA序列与地芽孢杆菌属(Geobacillus)内各种之间的同源性为98%以上.克隆得到DYth03的DNA聚合酶基因(DYth-pol),序列分析表明该基因全长为2 631 bp,G+C含量为55.5%,推测编码为876个氨基酸,与BstDNApolI的同源性最高(达98%).将该聚合酶基因克隆到pTTQ-h表达载体上,并在大肠杆菌DH1中进行表达.对纯化到的表达产物进行酶活性测定,结果表明该酶具有聚合酶活性和5’-3’外切酶活性.
A strain of thermophilic bacteria（DYth03） that can grow at 65℃ was isolated from the sediment of a hydrothermal vent field on the E53 site of the east Pacific Ocean.The 16S rDNA of DYth03 shared more than 98% sequence homology with related Geobacillus species.The gene encoding the DNA polymerase of DYth03（DYth-pol） was cloned and sequenced.Analysis indicated that the gene was 2 631 bp in length with a G＋C content of 55.5%,encoding an 876-amino-acid peptide,which was most similar to that of Bst DNApolI（98%）.The DYth-pol gene was cloned in the expression vetor pTTQ-h and expressed in E.coli DH1.The expressed product was purified and characterized.It showed 5＇-3＇ exonuclease and polymerase activity.
出处
《台湾海峡》
CAS
CSCD
北大核心
2011年第1期17-20,共4页
Journal of Oceanography In Taiwan Strait
基金
国家863计划资助项目(2007AA091407)
