摘要
目的探索用去细胞异种肌腱复合胰岛素样生长因子Ⅰ(IGF-Ⅰ)修复肌腱缺损的可行性提供理论依据,为肌腱组织工程的细胞生长支架提供实验依据。方法切取月龄(6个月)相同Leghorn鸡屈趾肌腱经化学去细胞处理后作为异种肌腱移植供体,健康成熟日本大耳白兔54只,建立双后肢跟腱中间束2 cm缺损模型,随机分为3组,每组18只。实验组为去细胞异种肌腱复合IGF-Ⅰ移植组(A组)、对照组Ⅰ为自体肌腱移植组(B组)、对照组Ⅱ为单纯去细胞异种肌腱移植组(C组)。术后伸直位管型石膏固定2周,术前3 d 10只兔及术后第3、7、14天每组10只兔取耳缘静脉血行血常规检测;2、4、9周每组取6只兔对标本行生物力学测定、组织学光镜检测及图像分析。结果 (1)在相同时期内,A、B、C三组血常规白细胞数两两比较差异无统计学意义(P>0.05)。(2)术后相同时间内三组生物力学两两比较,A组与B组之间无统计学差异(P>0.05),A、B组与C组之间差异有统计学意义(P<0.05),术后2周时肌腱最大抗拉力最差,9周时A、B两组最大抗拉力接近正常水平。(3)肌腱移植后总体上经历了细胞长入、胶原纤维再生、替代和再塑形的过程,术后相同时间内三组胶原纤维含量两两比较,A、B组与C组之间差异有统计学意义(P<0.05),A组与B组之间差异无统计学意义(P>0.05)。结论 (1)化学去细胞肌腱可明显降低其免疫原性,移植后无明显的免疫排斥反应发生,其力学强度亦能满足肌腱移植的需要。(2)化学去细胞异种肌腱复合IGI-Ⅰ修复肌腱缺损的效果优于单纯化学去细胞异种肌腱的修复效果。
Objective This experiment was conducted to explore the possibility of repairing tendon defect with chemical xenogeneic acellular tendon combined with IGF- I, and simultaneously provided an experimental foundation for cell scaffold of chemical xenogeneic acellular tendon considered as tendon tissue engineering. Methods The-same-age（six months） leghorn chicken＇s flexor tendons were treated in chemical acellular way as xenogeneic tendon transplant gaffs. Based on the experiment, we established the injured model that Double hindlimb Achilles＇ tendon in the middle beam had 2 crn gap. 54 Japan rabbits were divided into 3 groups randomly, 18 in each group. The experimental group I was transplant group treated by chemical xenogeneic aceUular tendon combined with IGF- I （group A）, control group I was autogenous tendon grafting （group B）, and control group II was single xenogeneic aceUular tendon grafting （group C）. They were immobilized straightly by plaster stone for two weeks. Blood test of 10 rabbits in each group were examined before operation 3 days and after 3, 7, 14 days. At 2, 4, 9 weeks, specimens of 6 rabbits in each group were evaluated by means of biomechanics test, engineering observation by Optical microscope and image analysis. Results （1） Within the same period, the differences among groups in leukocyte count of blood test were not significant （P〉0.05）. （2） Comparing biomechanics in three groups at the same time, we can see the little significant difference between group A and group B （P〉0.05）. In contrast, there wire significant differences between group A, B and group C （P〈0.05）. At the 2 weeks, tensile strength was the worst, but 9 weeks later it approached the normal level. （3） Atter tendon transplant, it experienced cell forming, collagen fibers regeneration, replacement and reshape. During the same period, when the collagencontent were compared, there were significant differences between group A, B and group C （P〈0.05） ,but there was no significant difference compared group A and group B （P〉0.05）. Conclusion （1）Chemical acellular tendon could obviously reduce immunogenicity and there was little immune rejection after transplant and could meet the needs of tendon mechanical strength. （2）Chemical xenogeneic acellular tendon combined with IGF- I had the better effect to repair than single xenogeneic acellular tendon.
出处
《中华临床医师杂志（电子版）》
CAS
2013年第17期95-99,共5页
Chinese Journal of Clinicians(Electronic Edition)
基金
内蒙古111人才工程和教育厅重点领域项目(ZL01008)
