摘要
目的初步探讨植物甾醇对人神经母细胞瘤SH-SY5Y细胞增殖和凋亡的影响。方法采用体外细胞培养的方法,用不同浓度的植物甾醇与细胞共同培养,倒置显微镜观察细胞形态,MTT法测定细胞存活率,流式细胞术测定细胞凋亡率。结果与对照组比较,从1μmol/L植物甾醇起即对细胞的存活率有明显影响,差异有统计学意义(P<0.01);植物甾醇浓度由低到高,随着浓度的增加,对细胞的形态影响越明显,突触变短,胞体萎缩甚至死亡;不同浓度的植物甾醇可引起细胞不同程度的早期凋亡。结论植物甾醇对体外培养的人神经母细胞瘤SHSY5Y细胞有潜在的毒性作用,能够抑制细胞增殖和引起细胞凋亡。
Objective It was discussed in the article about the effect of plant sterol on cell proliferation and apoptosis of SH-SYSY cell. Methods Using cell culture, SH-SYSY cells were treated with different concentrations of plant sterol. Morphology of the cells was analyzed by inverted microscope, cell survival rate by MTT assay, determination of cell apoptosis by flow cytometry. Results Compared with the control group, phytosterol concentration of 1 μmol/L, had a significant effect on cell survival rate （ P 〈 0. 01 ） ; Morphological comparison revealed increase with different concentrations of plant sterol including shorter synapses, cellular degeneration and atrophy; Early apoptosis was caused by different concentrations of plant sterol. Conclusion Phytosterols has the potential to be cytotoxic for neuron by inhibiting cell growth and inducing apoptosis.
出处
《中国食品卫生杂志》
北大核心
2013年第6期497-500,共4页
Chinese Journal of Food Hygiene
