摘要
目的 :了解磷脂酶C(PLC)和Gq蛋白亚单位 (Gaq)与原发性高血压即高血压病的相关性。方法 :分别以Gq蛋白α亚单位 (Gαq)多克隆抗体及PLC β1和PLC γ1单克隆抗体为探针 ,用免疫印迹 (Immunoblot)方法测定人动脉平滑肌层中Gαq、PLC β1、PLC γ1的相对含量。结果 :在人类动脉血管平滑肌层中未检测到PLC β1,但存在Gαq和PLC γ1。原发性高血压病人组 (n =9)和对照组 (n =15 )相比较 ,Gαq和PLC γ1均无显著性差异。结论 :在原发性高血压病人的发病过程中并无证明存在有Gαq和磷脂酶C信号途径的异常。
Aim: to know the relationship between phospholipase C(PLC) and essential hypertension. Method:To examine the relative content of G αq 、PLC β 1、PLC γ 1in arterial smooth muscle tissue using G αq polyclonal antibody and PLC β 1、PLC γ 1 monoclonal antibodies as probes and immunoblot hybridization. Result:PLC β 1 was not detected in human's arteries, but PLC γ 1 was found. As compared with controls( n =15), protein content of G αq or PLC γ 1 in arteries from hypertensive patients( n =9) had no significant change. Conclusion:May be it is because of the long administration of antihypertensive drugs in our cases, or the abnormality of PLC δ, whose mechanism of regulation is not clear and which is robustly expressed in arteries, or no role played by G αq and PLC in the pathogenesis of essential hypertension.
出处
《高血压杂志》
CSCD
2000年第4期312-314,共3页
Chinese Journal of Hypertension
基金
国家自然科学基金资助项目(编号:39270722)
浙江省(编号:392037)
