摘要
为建立藏猪颗粒细胞的体外培养和保存方法,从藏猪卵巢上采集卵泡的颗粒细胞,进行分离培养和冷冻复苏。结果显示:藏猪颗粒细胞形成单层需要6～7d;细胞冷冻复苏后仍可进行正常生长,冻存前后活率都在90%以上;细胞生长曲线呈"S"型,接种后,经历2d的潜伏期,第3天细胞开始迅速生长,进入对数生长期,到第8天细胞开始发生接触抑制,进入生长缓慢的平台期。表明建立的藏猪颗粒细胞的体外培养和保存方法可行有效。
In order to establish a set of methods for the in v itro culture and storage of granule cells of Tibet pigs ,granule cells were collected from the ovary of Tibet pigs .The results showed that the formation of granule cells monolayers needed 6-7 days and the cells could be passaged after freezing-thawing .The survival rate of cells before or after freezing was above 90% .The growth curve was basically of the“S”type ,and its incubation period ,logarithmic grow th phase ,and plat-eau were about 0-2 d ,3-7 d ,and 8-12 d after the cells inoculation ,respectively .This indicated that the method for in vitro culture and save of granule cells of Tibet pigs was successfully estab-lished .
出处
《河南农业科学》
CSCD
北大核心
2013年第11期141-144,共4页
Journal of Henan Agricultural Sciences
基金
科技部国家科技支撑计划项目(2012BAD03B03)
西藏自治区强基惠民科研项目(2013)
关键词
藏猪
颗粒细胞
体外培养
Tibet pig
granule cells
in vitro culture
