摘要
探讨信号转导和转录激活子 ５（ＳＴＡＴＳ）信号转导途径在受辐射 ＫＧ－１细胞中对细胞 周期的调控作用。通过转染ＳＴＡＴ ５基因的显性负突变体（ＤＮ－ＳＴＡＴ ５）阻断ＪＡＫ／ＳＴＡＴ的信 号传递后， 瞬间转染 ｃｙｃｌｉｎ Ｄ１基因及 ｃｙｃｌｉｎ Ｂ１基因，观察 ｃｙｃｌｉｎＤ１蛋白及 ｃｙｃｌｉｎ Ｂ１蛋白对 受辐射细胞周期阻滞的影响作用。转染 ＤＮ－ＳＴＳＡＴ５基因的受辐射 ＫＧ－１细胞即使用粒－巨噬 细胞集落刺激因子（ＧＭ－ＣＳＦ） 刺激亦不能跳出 Ｇ１期阻滞； 瞬间转染 ｃｙｃｌｉｎ Ｄ１基因及 ｃｙｃｌｉｎ Ｂ１基因分别能促进受辐射细胞跳出 Ｇ１期和 Ｇ２期阻滞。 ＧＭ－ＣＳＦ所激活的 ＪＡＫ／ＳＴＡＴ 信 号转导途径通过促进周期蛋白 ｃｙｃｌｉｎ Ｄ１及 ｃｙｃｌｉｎ Ｂ１的表达而对受辐射细胞周期进行调控。
This study investigated the role of the JAK/STAT signaling pathway regulating cell cycle progression in the irradiated KG-1 cells. By permanent transfecting the cells with DN-STAT5 cDNA to block the JAK/STAT signaling pathway and then transient transfecting with cyclin D1 or cyclin B1 cDNA, the effects of cyclin D1 protein and cyclin B1 protein on the cell cycle progression were examined. Results showed that after irradiation with 8Gy 60Co r rays, the irradiated KG-1 cells transfected with only DN-STAT5 cDNA can not recover form the G1 arrest, even though GM-CSF was added. Meanwhile, the cells transfected with both the DN-STAT5 cDNA and cyclin D1 cDNA or cyclin B1 cDNA can recover from the G1 arrest or the G2 arrest to a great extent. Thus, it was proved indirectly that the JAK/STAT signaling pathway activated by GM-CSF regulated the cell cycle progression through cyclin D1 and cyclin B1 protein.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
2000年第4期299-303,共5页
Journal of Radiation Research and Radiation Processing
