摘要
用荧光光谱法研究了298 K下Tris-HCl缓冲溶液(pH=7.1)中不同离子强度下,季铵盐类表面活性剂N-十四烷基-N-羟乙基-N,N-二甲基溴化铵(THDAB)与牛血清白蛋白(BSA)的结合作用.考察了离子强度对二者结合作用的影响,分别用Stern-Volmer方程、位点结合模型探讨了THDAB在浓度较低区域与BSA的作用机制,用位点结合模型计算了不同离子强度下THDAB和BSA结合反应的结合常数K A和结合位点数n.结果表明:随离子强度的增大,THDAB与BSA的结合作用减弱.
The interaction of N-tetradecyl-N-hydroxyethyl-N,N-dimethyl ammonium bromide and bovine serum albumins in Tris-HC1 buffer solution （pH = 7.1 ） under different ionic strength was studied by fluores- cence spectroscopy at 298 K.The influence of inonic strength on the interaction between THDAB and BSA within a relatively low concentration range was examined.The interaction mechanism between THDAB and BSA at a relatively low concentration range was discussed using the Stern-Volmer equation.The binding con- stant and the number of binding site of the binding reaction between THDAB and BSA under different ionic strength were calculated using site binding model.Resuh indicated that along with the enhancement of ionic strength,the interaction of THDAB and BSA was weakened.
出处
《浙江外国语学院学报》
2013年第4期7-11,共5页
Journal of Zhejiang International Studies University
