摘要
通过控制不同酶解时间制备麦胚蛋白酶解产物(WGPIH),结合其体外抗氧化活性,采用MTT法比较研究WGPIH对H2O2诱导PC12细胞氧化损伤模型的抗氧化作用,并利用扫描电镜进一步观察PC12细胞表面微观结构的变化。结果表明:酶解时间为240 min的产物(WGPIH5)在不同抗氧化模型中均呈现最强的活性,Fe2+螯合能力的IC50为(0.37±0.05)mg/mL;WGPIH5质量浓度为5mg/mL时,TEAC值(Trolox)可达(0.81±0.01)mmol/L,可有效抑制亚油酸体系的自氧化;WGPIH5质量浓度为1 mg/mL时,可使H2O2诱导损伤的PC12细胞存活率提高32.85个百分点;扫描电镜观察WGPIH5可保持PC12细胞膜的完整性,减少凋亡小体的出现。
Wheat germ protein isolate hydrolysates （WGPIH） were prepared under different enzymolysis time and the antioxidant effects of different WGPIH against the H202 -induced oxidative stress in PC12 cells were compared through MTr method combined with their antioxidant activities in vitro, and the changes of the PC12 cells surface microstructures were observed by SEM. The results showed that the hy- drolysate WGPIH5 produced by hydrolyze for 240 rain possessed the highest antioxidant activity, its IC5o value for ferrous iron - chelating activity was（0.37 ＋_0.05 ） mg/mL;TEAC value （Trolox） reached（0.81 ＋_ 0.01 ）mmol/L with 5 mg/mL WGPIH5; the autooxidation of linoleic acid was significantly inhibited by WGPIH5;WGPIH5 was able to attenuate H202 -induced oxidative stress in PC12 cells in some extend, and the cell viability of PC12 cells increased by 32. 85 percentage point with 1 mg/mL WGPIH5; SEM results showed that WGPIH5 could keep the integrity of PC12 cells membranes and reduce the appearance of apototic bodies.
出处
《中国油脂》
CAS
CSCD
北大核心
2013年第11期18-22,共5页
China Oils and Fats
基金
国家自然科学基金资助项目(31101384)
江苏省自然科学基金项目(BK2011041)
关键词
麦胚蛋白酶解物
抗氧化活性
PC12细胞
氧化应激
wheat germ protein isolate hydrolysate
antioxidant activity
PC12 cell
oxidative stress
