摘要
目的优化树莓总多酚的微波辅助提取工艺,考察树莓总多酚提取物的体外抗氧化活性。方法在单因素试验基础上,以总多酚提取率为考察指标,通过正交试验设计优化微波辅助提取树莓总多酚的工艺条件,采用DPPH和ABTS自由基清除法对树莓总多酚提取物进行体外抗氧化活性评价。结果最佳提取工艺为乙醇体积分数50%、微波提取温度80℃、提取时间10 min、液料比20 mL·g^(-1),在此条件下,总多酚提取率的质量分数达到0.408%。DPPH和ABTS自由基清除试验表明,树莓总多酚提取物具有较强的抗氧化活性。结论微波辅助提取技术适于提取树莓中的总多酚,优化后的提取工艺高效简便,提取物具有较强的抗氧化活性。
Objective To optimize the process for the microwave-assisted extraction（MAE） of phenolic com- pounds and evaluate the antioxidant activity of the MAE extract by scavenging capability. Methods The effects of ethanol concentration, extraction temperature, extraction time and liquid to solid ratio on phenolic compounds yield were investigated by single factor method, and then orthogonal design was employed to op- timize condition. In addition, the antioxidant activities of the MAE extract were evaluated in vitro by scaven- ging capability of DPPH and ABTS free radical. Results The optimal condition obtained was as follows ： eth- anol concentration was 50% ,microwave extraction time was 10 rain, extraction temperature was 80 ℃, and ratio of liquid to solid was 20 mL·g ^-1, the extraction yield was 0. 408 %. DPPH and ABTS radical scaven- ging experiment showed that the phenolic compounds had significant antioxidant activity. Conclusions MAE is proved to be an effective method for phenolic compounds extraction and the extract of MAE shows higher antioxidant activity than trolox, which makes this fruit a good source of antioxidant in health-care food and pharmaceutical industry.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2013年第11期906-910,共5页
Journal of Shenyang Pharmaceutical University
关键词
树莓
总多酚
微波辅助提取
正交设计
DPPH法
ABTS法
raspberry
total phenols
microwave-assisted extraction
orthogonal design
DPPH method
ABTS method
