摘要
乙酰乳酸合成酶 (ALS)是除草剂主要作用靶标 ,新型除草剂农美利 (bispyribac sodium)即是通过抑制ALS的活性使支链氨基酸的生物合成受阻而达到除草目的。作者利用ALS脱羧产物 3 羟基丁酮与肌酸及甲萘酚形成的复合物 ,采用比色法测定ALS活性和农美利的抑制作用 ,以明确农美利对水稻和稗草的选择性机理。研究结果表明 ,水稻和稗草体内ALS酶促反应复合产物的吸收峰为 52 0nm ;ALS活性在不同水稻品种和不同杂草体内各不相同 ,不同水稻品种依次为汕优 63、秀水 11>祥湖 84 >嘉育 2 93,不同杂草依次为稗草、鳢肠 >异型莎草 >千金子。体内法测定农美利对植物体内ALS的抑制程度结果显示 ,水稻ALS受抑制快于稗草 ,恢复也快 ;不同水稻品种ALS以嘉育 2 93受抑制较快 ,秀水 11抑制程度较大 ,而祥湖 84和汕优 63受抑制慢且程度低。体外法测定不同化合物对ALS抑制程度 。
Acetolactate synthase (ALS) is the main target of herbicides and bispyribac sodium prevents biosynthesis of branched chain amino acids by inhibition of ALS.In this paper,the activity of ALS was determined by an acetoin creatine naphthol complex on spectrophotometer and inhibition of bispyribac sodium to ALS in rice and barnyardgrass assessed.The results showed absorb peak of the complex for rice and barnyardgrass is 520 nm.Rice varieties Xianyou 63 and Xiushui 11 have the highest ALS activity,Jiayu 293 has the lowest one,and Xianhu 84 with the medium.For different weeds,the order of ALS activity from high to low is Echinochloa crusgalli,Eclipta prostrata,Cyperus difformis,Leptochloa chinensis. By in vivo test,ALS is prevented by bispyribac sodium and then recovered quicker in rice than in barnyardgrass.For rice varieties,ALS is repressed quickly in Jiayu 293,strongly in Xiushui 11,whereas in Xianhu 84 and Xianyou 63 the herbicide has slow and week inhibition to ALS.By in vitro test,the inhibition of bispyribac sodium to ALS is stronger than pyanchor.
出处
《浙江农业学报》
CSCD
2000年第6期303-307,共5页
Acta Agriculturae Zhejiangensis
基金
浙江省"九五"重大科技项目 !(96 110 2 2 42 )
关键词
乙酰乳酸合成酶
农美利
选择性机理
除草剂
稻田
acetolactate synthase(ALS)activity determination
bispyribac sodium
pyanchor
selectivity mechanism
