摘要
随着石油能源的开采以及广泛应用,水体环境石油污染问题日益严重,找到高效的石油降解菌株成为水体石油污染生物修复的一个重要研究方向。文章研究从污染水环境中分离筛选到SY2、J7、H2 3株具有较好石油降解能力的菌株,经生理生化及16Sr DNA分子鉴定,分别为枯草芽孢杆(Bacillus subtilis)、恶臭假单胞菌(Pseudomonas putida)、铜绿假单胞菌(Pseudomonas aeruginosa)。分别考察了不同温度、pH、盐度、原油浓度、培养降解时间、过氧化氢深度氧化6个因素对上述菌株石油烃降解效果的影响。结果表明:最优条件下,SY2石油烃降解率最高可达61.27%,J7达68.40%,H2达83.64%。气相色谱分析石油烃降解前后的全烃组成分析进一步证实其均具有良好的石油烃降解能力。为基于微生物的石油水体污染生物修复提供了应用基础。
With oil energy mining and widely application, oil pollution in water becomes more and more serious, it is an im- portant research direction to find high efficient oil degradation bacteria for bioremediation. This research is about three isolat- ed strains SY2, J7, H2 from external oil polluted water, which separately indentified as Bacillus subtilis, Pseudomonas putida, Pseudomonas aeruginosa through the physiological and biochemical and 16Sr DNA molecular identification. Effects of six factors such as different temperature, pH, salinity, crude oil concentration, training time and hydrogen peroxide depth oxida- tion on petroleum hydrocarbon degradation about the three strains were studied. Results showed that with the optimal condi- tions, the highest oil degradation rate of SY2 can amount to 61.27%, and J7, H2 amount to 68.40%, 83.64% respectively. GC analysis of total hydrocarbon composition of the oil before and after the biodegradation indicated that the strains have effec- tive oil degradation ability. The study would provide application foundations for bioremediation of oil water pollution.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2013年第10期101-106,共6页
Environmental Science & Technology
基金
北部湾区域重大环境突发性污染事件应急技术开发与示范子项目子课题(2009AA06A416)
关键词
石油降解菌
分离
鉴定
降解特性
气相色谱
petroleum degradation bacteria
isolation
identification
degradation characteristics
GC
