摘要
目的 :探讨采用自制的抗SAⅠ Ⅱ和抗SPAa 的单克隆抗体检测人牙菌斑中变链球菌c,d血清型的可行性。方法 :应用菌落免疫印迹技术 ,采用自制的抗SAⅠ Ⅱ和抗SPAa 的单克隆抗体WC2E10c和WC3A6d ,检测人牙菌斑中的变链球菌。结果 :肉眼观阳性菌落为深褐色 ,菌落间隙清楚 ;阴性菌落呈白色。经形态学、血清学、生化反应及SDS PAGE鉴定均证明两种菌落检出的准确性。结论 :自制的单克隆抗体对人牙菌斑中变链球菌c ,d血清型的检出显示高度特异性。
Objective: To explore the possibility of detecting the distribution of Mutans Streptecocci in human dental plaque by Monoclonal antibody (WC2E10c,WC3A6d) against SAⅠ/Ⅱ and SP\+A\-a of Mutans Streptococci, which was prepared in our lab. Methods: 60 subjects were divided into the exprimental group and the control group. The distributions of Mutans Streptococci (serotype c and d) in dental plaques were detected in 60 subjects by the clonal blot technique. Positive colonies were examined by bacterial morphology, serology, biochemistry and SDS PAGE. Results: Bacterial coloning was significantly different between the experimental group and the control group with eye observation. Colonies of the experimental group were deep brown and easily differentiated, but colonies of controt group were white. Brown colonies were proved to be Mutans Streptococci (serotype c and d) by using assay of bacterial morphology, serology, biochemistry and SDS PAGE, but white colonies were not. Conclusion: McAbs (WC2E10c, WC3A6d) were shown high specificity in Mutans Streptococci (Serotype c and d) of human dental plaques. \;
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2000年第6期383-385,共3页
West China Journal of Stomatology
基金
国家教委博士点基金!资助 (编号 880 114 )
关键词
变链球菌
单克隆抗体
细菌检测
牙菌斑
Mutans Streptococci\ \ Monoclonal antibody\ \ human dental plaque
