摘要
目的 制备99mTc-CL3-Bt并对其质量控制、生物分布进行研究。方法 将单抗CL3生物素（Bt）化，以2-巯基乙醇（2-ME）还原后以直接法进行99mTc 标记。以细胞结合实验测定并比较99mTc-CL3-Bt 和99mTc-CL3的免疫活性；然后将两种标记物注入荷大肠癌裸鼠模型，6 h后处死裸鼠，计算并比较99mTc-CL3-Bt 和99mTc-CL3在体内的每克组织摄取分数（ID%/g）值和肿瘤ID%/g/其它组织ID%/g（T/NT）比值。结果99mTc-CL3-Bt和99mTc-CL3的细胞结合率分别为90%、94%；在瘤体的ID%/g值分别为（6.3±1.1）、（6.7±0.9），二者无显著差异（t=0.6293, P>0.05）；瘤/血比值分别为（0.70±0.24）、（0.80±0.12），二者亦无显著差异（t=0.8333, P>0.05）。结论 99mTc-CL3-Bt免疫活性高，能特异性被肿瘤摄取，可广泛用于临床放射免疫显像研究。
Objective To prepare 99mTc-labeled biotinylated CL3 (99mTc-CL3-Bt) and study its quality control and biodistribution in rats. Methods Monoclonal antibody (Mab) CL3 binding to biotin was reduced with 2-mercaptoethanol (2-ME) and labeled directly with 99mTc to produce 99mTc-CL3. The immunoactivity of 99mTc-CL3-Bt and 99mTc-CL3 was measured by cell binding test and the results compared; The 2 labeled antibodies were injected into nude mice bearing colorectal cancer xenograft via tail vein at the dosage of 99mTc-CL3-Bt and 99mTc-CL3 respectively. The mice were put to death 6 h later and the percentage of absorbed dose per gram tissue (ID%/g) and its tumor to non-tumor ratio (T/NT) were measured. Results The cell binding rates of 99mTc-CL3-Bt and 99mTc-CL3 were 90% and 94%, and their ID%/g of tumor averaged 6.3±1.1 and 6.7±0.9 respectively, which were statistically comparable (t=0.6293,P>0.05); the ratios of the ID%/g in the tumor to that in the blood were 0.70±0.24 and 0.80±0.12 respectively, showing no significant difference (t=0.8333, P>0.05), either. Conclusion With out decrease of immunoactivity after its preparation, 99mTc-CL3-Bt can be absorbed into the tumor specifically and is applicable in the clinical research of radioimmunoimaging.
出处
《第一军医大学学报》
CSCD
2000年第6期515-516,共2页
Journal of First Military Medical University
