摘要
目的研究测定胰腺癌Patu8988细胞的18F-氟代脱氧葡萄糖(FDG)细胞结合率方法和吉西他滨化疗后对胰腺癌Patu8988细胞摄取18F-FDG的影响。方法在不同条件下测定胰腺癌Patu8988细胞的18F-FDG细胞结合率,细胞数量为5×104～1×107/瓶,18F-FDG放射性活度为1.85～29.6kBq,反应时间为20～120min,葡萄糖浓度为0～11.1mmol/L。采用四甲基偶氮唑盐(MTT)法测定加入不同剂量(0～6mmol/L)吉西他滨10μl24h后细胞抑制率。测定加入不同剂量(0～60mmol/L)吉西他滨100μl24h后18F-FDG细胞结合率。结果当每瓶1×106个细胞、加入3.7kBq18F-FDG、葡萄糖浓度为5.55mmol/L、作用100min时,细胞结合率可达到(60.60±3.05)%。加入0、5、20、40和60mmol/L吉西他滨24h后,细胞结合率分别为(58.35±2.19)%、(56.34±1.56)%、(48.92±5.91)%、(39.14±7.40)%、(29.67±4.41)%。细胞结合率随吉西他滨剂量增加而下降,两者成负相关(r=-0.928,P<0.01)。给吉西他滨24h后,MTT实验抑制率与细胞结合抑制率成正相关(r=0.856,P<0.01)。结论吉西他滨作用24h后引起胰腺癌Patu898818F-FDG细胞结合率下降,有望用18F-FDG显像早期观测吉西他滨对胰腺癌的疗效。
Objective To investigate the binding characteristic of 18F-fluorodeoxyglucose（ FDG） with human pancreatic carcinoma cell Patu 8988 and the effect of gemcitabine in the uptake of 18F-FDG on Patu 8988. Methods The binding efficiency of 18F-FDG was measured under different conditions： 5 × 104 ～ 1 × 107 cells; 1. 85 ～ 29. 6 kBq 18F-FDG; 0 ～ 11. 1mmol/L glucose levels; 20 ～ 120 min incubation in 37℃. The proliferation inhibition effect and the binding efficiency of 18F-FDG were detected at the 24th hour after administration of gemcitabine. Results The binding efficiency was （ 60. 60 ± 3. 05） % at the optimum binding condition of 1 × 106cells,3. 7kBq 18F-FDG,5. 55mmol/L glucose and 100min incu-bation at 37℃. The binding efficiency was （ 58. 35 ± 2. 19） % ,（ 56. 34 ± 1. 56） % ,（ 48. 92 ± 5. 91） % , （ 39. 14 ±7. 40） %,（ 29. 67 ±4. 41） % at the 24th hour after administration of 0,5,20,40,60mmol/L gemcitabine. The binding efficiency was negatively correlated with the dose of gemcitabine （ r =-0. 928, P 0. 01） . The binding inhibition efficiency was positively correlated with the proliferation inhibition （ r = 0. 856,P 0. 01） . Conclusion The binding efficiency of 18F-FDG with human pancreatic carcinoma cell Patu 8988 decreased at the 24th hour after administration of gemcitabine.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2010年第5期1010-1013,1020,共5页
Suzhou University Journal of Medical Science
基金
卫生部核医学重点实验室开放课题(KF200904)
2008年度苏州大学附属第一医院联邦优秀中青年医师培养基金(LB0815)
