摘要
本研究利用10对多态性丰富的微卫星引物，结合荧光一多重PCR技术、STR分型技术，对皖西白鹅、豁眼鹅、浙东白鹅等我国9个地方鹅品种（共540只）群体进行微卫星分型。根据等位基因频率，计算有效等位基因数（肫）、群体杂合度（He）等群体遗传学参数，分析群体内和群体间的遗传变异。结果表明：在10个座位上共检测到89个等位基因；群体的平均胁为0．5785，平均P1C值为0．5949，其中马岗鹅遗传变异最大。群体间遗传分化极显著，遗传分化系数为0．112（P〈0．001），并且10个座位都极显著（P〈0．001）地贡献于这一结果。从遗传距离为0．0454～0．2342、Nm变异为0．7404～5．3806。聚类分析显示这9个鹅群体可分为4个类群。
Combining the technique of multiple PCR and STR, 10 pairs of polymorphic microsatellite markers were selected to detect the genetic diversity and phylogenetic relationship among 540 individuals of 9 Chinese domestic goose breeds. Alleles were scored using GeneMapper v4.0 software. The genetic diversity was disclosed by calculating the allele frequency, heterozygosity （He）, effective number of alleles （Ne）, polymorphism information content （PIC） and Ds genetic distance. The main results were showed that a total of 89 alleles were detected in the 10 loci and the overall expected heterozygosity of all populations and PIC of all loci were 0.5785±0.012 and 0.5949, respectively. Magang goose had the highest genetic variation. The genetic differentiation among populations had reached to 0.112 （P〈0.001）, which was very significant for the whole population, all loci were contributed significantly （P〈0.001） to this differentiation. Ds values varied between 0.0454 and 0.2342. The Nm value was ranged from 0.7404 5. 3806. The phylogenetic relationship was analyzed and an un-rooted consensus tree was constructed, and then the 9 indigenous goose and divided them into 4 groups.
出处
《中国畜牧杂志》
CAS
北大核心
2013年第21期1-6,共6页
Chinese Journal of Animal Science
基金
现代农业产业技术体系建设专项资金(nycytx-45-04)
关键词
STR分型
遗传多样性
聚类分析
鹅
indigenous goose
STR genotyping
genetic diversity
cluster analysis
