摘要
目的 :对维持性血液透析 (HD)患者外周血中性粒细胞 (PMN)白介素 - 1受体拮抗物 (IL- 1ra)基因表达和吞噬及杀菌功能进行研究 ,并探讨二者的相关性。 方法 :用 RT- PCR方法检测 HD患者外周血 PMN ic IL- 1ra(胞内型 )和 s IL- 1ra(分泌型 )基因表达 ;通过菌落计数方法观察 PMN对金黄色葡萄球菌 (金葡菌 )吞噬和杀菌功能的改变。 结果 :HD患者 PMN中可检测到 5 12 bp(ic IL- 1ra)和 146 bp(s IL- 1ra)的 RT- PCR产物 ;HD患者 PMN对金葡菌的吞噬和胞内杀菌能力均较正常对照明显下降 (P<0 .0 1或 P<0 .0 5 )。结论 :HD患者 PMN可自发表达 ic IL- 1ra和 s IL- 1ra,这种预激活状态与其对金葡菌的吞噬和杀灭作用下降相平行。
Objective: To investigate the function and IL 1ra gene expression of neutrophils in hemodialysis (HD) patients. Methods: Gene expression was studied by reverse transcribe polymerase chain reaction (RT PCR) from untreated neutrophils in healthy controls and HD patients. Phagocytosis and bactericidal assays decreased in the number of viable extracellular and intracellular bacteria by colony counts. Results: Freshly isolated neutrophils spontaneously expressed icIL 1ra, sIL 1ra mRNA in HD patients. Phagocytosis and intracellular killing of neutrophils in HD patients impaired obviously when exposed to staphylococcus aureus in comparison with healthy controls. Conclusion: Untreated neutrophils in HD patients spontaneously express icIL 1ra and sIL 1ra mRNA, which is consistent with the suppressed phagocytosis and intracellular killing capacity. [
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2000年第11期1032-1034,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金资助项目 ( 3 92 70 3 4 5 )
关键词
中性粒细胞
血液透析
IL-1RA
吞噬作用
neutrophils
hemodialysis
receptor antagonists, IL 1
phagocytosis
antisptics
