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大鼠脑缺血再灌注对白质的损伤作用

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摘要 目的观察和评价大鼠脑缺血再灌注诱发白质少突胶质细胞和髓鞘的损伤变化。方法按照随机原则将实验动物随机分为假手术组(10只),再灌注3 d组(15只)。采用线栓法制备大鼠大脑中动脉闭塞缺血再灌注(middle cerebral artery occlusion reperfusion,MCAO/R)模型,应用免疫组织化学染色检测髓鞘碱性蛋白(myelin basic protein,MBP)的表达与分布;末端脱氧核苷酸转移酶介导的d-UTP切口末端标记技术(TdT2-ediated d-UTP nickend labeling technique,TUNEL法)检测凋亡细胞。结果脑缺血再灌注后,动物均表现为神经行为功能障碍;与假手术组相比,患侧脑白质MBP阳性分布减少,可见多量凋亡细胞。结论脑缺血再灌注可对脑白质造成显著损伤,表现为少突胶质细胞的凋亡,以及髓鞘的损伤。
作者 郑晓燕 张良成 周琳瑛 高美钦 郭永正
机构地区 福建医科大学附属协和医院麻醉科 福建医科大学病理学系
出处 《中国实用医药》 2013年第31期240-242,共3页 China Practical Medicine
关键词 脑缺血 再灌注损伤 少突胶质细胞 髓鞘 髓鞘碱性蛋白
分类号 R743.3 [医药卫生—神经病学与精神病学]
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参考文献9

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中国实用医药
2013年 第31期

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