摘要
目的探讨低分子量酪氨酸磷酸酶（LMW—PTP）在子宫颈鳞癌中的表达及其临床意义。方法采用RT—PCR及免疫组化法分析HaCaT、C33A、SiHa和CaSki细胞系中LMW，PTP的表达情况。免疫组织化学法检测正常宫颈患者19例，宫颈上皮内瘤变（CIN）I期患者18例、CINⅡ～Ⅲ患者46例和宫颈鳞癌患者76例的组织切片中LMW—PTP的表达。结果LMW—PTPmRNA及蛋白在C33A、SiHa及CaSki宫颈癌细胞系中表达明显高于人永生化正常表皮细胞系HaCaT；临床组织样本中，正常宫颈上皮LMW—PTP几乎不表达，在正常宫颈上皮到CIN继而发展成宫颈癌的进展过程中LMW—PTP的表达逐渐升高，差异有统计学意义（P〈0．05）；低分化宫颈癌组织中LMW—PTP表达高于中分化组织（P=0．025），中分化宫颈癌组织中LMW—PTP的表达高于高分化组织（P=0．045）；宫颈癌组织中LMW—PTP蛋白的表达与组织学分级程度和是否有淋巴结转移密切相关（P〈0．05）。结论LMW—PTP在宫颈癌的进展过程中起重要作用，且与组织分化程度和是否有淋巴转移密切相关，提示LMW—PTP的表达有助于判断宫颈癌患者的预后。
Objective To detect the expression of low molecular weight protein tyrosine phosphatase （LMW-PTP） proteins and its clinical significance in cervical cancer. Methods The expressions of LMW-PTP were determined by RT-PCR and immunohistochernieal assay in HaCaT, C33A, SiHa and CaSki. A series of samples, including 19 cases of normal cervical tissues, 18 cases of cervical intracpithelial neoplasia I phase, 46 cases of cervical intraepithelial neoplasia lI or m phase, 76 cases of squamous cell carcinoma （SCC） , were used to examine LMW-PTP immunohistochemical staining and statistical analysis. Results RT-PCR and Western blot results showed that expression of LMW-PTP in C33A, SiHa and CaSki were higher than expression of LMW-TFP in HaCaT. The results showed that LMW-PTP was barely observed in the normal cervical epithelial cells. With the progression of normal cervical epithelial cells to CIN and cervical cancer, the expression levels of LMW-PTP were increased. There was significantly difference for LMW-PTP expression among normal cervical tissues, CIN, cervical cancer （P 〈 0.05 ）. 20 The expression of LMW-PTP in poorly differentiated cervical cancer was higher than expression of LMW-PTP in moderately differentiated cervical cancer which was higher expression of LMW-PTP than high differentiation of cerwith the progression of cervical carcinogenesis and development of cervical cancer. In addition, the association LMW-PTP with lymph node metastasis and histological grade of SCC suggests that LMW-PTP can be a potential therapeutic target of SCC patients.
出处
《安徽医科大学学报》
CAS
北大核心
2013年第12期1522-1526,共5页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81001168)
安徽省科技攻关计划项目(编号:08010302101)
