摘要
采用常规组织分离法分离得到2个柱花草炭疽病菌致病性菌株,利用ITS通用引物ITS1/ITS4对炭疽菌rDNA-ITS进行扩增,电泳检测得到约500 bp大小的目的条带,经过生物信息学分析2个菌株均为刺盘孢属胶胞炭疽病菌,且两者之间同源性达到99%,对其侵染能力进行检测,5个柱花草品系的抗病性有所不同。
Anthracnose is the main plant disease of Stylosanthes. Identification of pathogen of the disease is par- tieularly important. Two pathogenic strains of anthracnose were separated by using conventional tissue isolation method, and the rDNA-ITS of the pathogens were amplified by using universal primers ITS1/ITS4. The electro- phoresis results showed that the target band was 500 bp approximately. Bioinformatics analysis showed that the two strains were clustered into Colletotrichum gloeosporioides and had 99% homology. Five Stylo species were in- oculated with the two pathogenic strains, and the result showed that these 5 stylo species were different in toler- ance to anthracnose.
出处
《热带生物学报》
2013年第3期220-224,共5页
Journal of Tropical Biology
基金
"国家牧草产业技术体系"之"热带牧草育种"(CARS-35)
