摘要
目的 评价长期糖皮质激素给药对非去极化肌松药诱发大鼠膈肌肌松效应的影响.方法 雄性SD大鼠40只,8周龄,体重180 ～ 220 g,采用随机数字表法,将其分为2组（n=20）：对照组和地塞米松组.地塞米松组每天腹腔注射地塞米松0.6 mg/kg,1次/d,连续注射14 d；对照组每天腹腔注射等容量生理盐水,1次/d,连续注射14 d.最后1次注射药物24h时,分离左膈神经-半膈肌,采用累积给药的方法进行实验,绘制d-筒箭毒碱的浓度-效应曲线,计算半数抑制浓度（IC50）及其95％可信区间.分离右膈肌,采用RT-PCR法测定胎儿型乙酰胆碱受体（nAchRγ） mRNA和成人型乙酰胆碱受体（nAchRε） mRNA的表达.结果 与对照组比较,地塞米松组浓度-效应曲线右移,IC50升高,nAchRγmRNA和nAchRεmRNA的表达上调（P＜0.05）.结论 长期糖皮质激素给药可减弱非去极化肌松药诱发大鼠膈肌肌松效应,其机制可能与nAchR mRNA表达上调有关.
Objective To evaluate the effect of long-term glucocorticoid administration on nondepolarizing muscle relaxants-induced neuromuscular blockade in rat diaphragma in vitro.Methods Forty adult male SpragueDawley rats,aged 8 weeks,weighing 180-220 g,were randomized into 2 groups （n =20 each）：control group （group C） and dexamethasone group （group D）.In group D,dexamethasone 0.6 mg/kg was injected intraperitoneally once a day for 14 consecutive days,while the equal volume of normal saline was injected instead of dexamethasone in group C.The left phrenic nerve-hemidiaphragm was removed at 24 h after the last injection to evaluate the effect of d-tubocurarine.Different concentrations （0-10 μmol/L） of d-tubocurarine were added.The d-tubocurarine concentration-effect curve was drawn.The half inhibitory concentration and 95 % confidence interval of d-tubocurarine were calculated.Right diaphragma was removed for determination of the expression of embryonic nicotinic acetylcholine receptor （nAChRγ） mRNA and adult nicotinic acetylcholine receptor （nAChRε） mRNA by RTPCR.Results Compared with group C,the concentration-effect curve was shifted to the right,the half inhibitory concentration was significantly increased,and the expression of nAChRγ mRNA and nAChRε mRNA was up-regulated in group D （P 〈 0.05）.Conclusion Long-term glucocorticoid administration can weaken nondepolarizing muscle relaxants-induced neuromuscular blockade in rat diaphragma in vitro,and up-regulation of nAChR mRNA expression may be involved in the mechanism. Keywords：Glucocorticoids ; Neuromuscular nondepolarizingagents ; Neuromuscular blockade
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2013年第9期1053-1055,共3页
Chinese Journal of Anesthesiology
关键词
糖皮质激素类
神经肌肉非去极药
神经肌肉阻滞
Glucocorticoids
Neuromuscular nondepolarizlngagents
Neuromuscular blockade
