摘要
目的考察复方蒲黄提取物对肾炎性模型大鼠血尿及肾功能的影响,对复方蒲黄提取物进行药效学评价,为开发医院制剂复方蒲黄颗粒提供实验基础。方法采用免疫原牛血清白蛋白(BSA)灌胃、四氯化碳(CCl4)皮下注射、脂多糖(LPS)尾静脉注射建立肾炎性血尿大鼠模型后,高、中、低剂量复方蒲黄提取物灌胃给药6周,分别于第4周、第6周测定大鼠血肌酐(SCr)和尿素氮(BUN)浓度、血清总蛋白(TP)、24 h尿蛋白、尿肌酐定量、尿畸形红细胞计数,计算内生肌酐清除率(CCr)。结果蒲黄组第4、6周分别与模型对照组比较,能明显减少尿中畸形红细胞个数、24 h尿蛋白、尿肌酐定量,降低血肌酐和尿素氮值,提高血清总蛋白以及内生肌酐清除率,差异有显著性(P<0.01或P<0.05)。结论复方蒲黄提取物可以减少肾炎性血尿模型大鼠的血尿、蛋白尿症状,改善肾功能各项指标,以高剂量组作用效果最佳。
OBJECTIVE To investigate the effect of compound pollen typhae extract on nephritis hematuria and renal function in rats, the pharmacodynamics evaluation laid a foundation for the development of hospital preparations compound pollen typhae granule. METHODS The rat model of renal hematuria was induced by immunogen bovine serum albumin （BSA） garages, carbon tetrachloride （ CC14 ） subcutaneous injection, and lipopolysaccharide （LPS） tail vein injection. Intragastric administration of high, medium and low-dose compound pollen typhae extract was performed for 6 weeks. Testing concentration of serum creatinine （SCr） , urea nitrogen （BUN） and total protein （TP） in rat blood, and 24 h urine protein, urine creatinine quantitative, urine deformed erythrocyte number were detected in the fourth week and sixth week respectively. RESULTS Compound pollen typhae extract reduced the urine deformed erythrocyte number, 24 h urinary proteins quantitative, cut down SCr and BUN, and increased TP and CCr, there are significant differences（P 〈0. 01 or P 〈 0. 05）. CONCLUSION Compound pollen typhae extract is effective in the treatment of glomerulonephritis hematuria in rat model through improving renal function, with high dose group of best effect.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2013年第22期1919-1923,共5页
Chinese Pharmaceutical Journal
基金
浙江省中医药管理局项目(2010ZA063
2011ZB063)
浙江省药学会医院药学专项科研基金项目(2012ZYY01)
关键词
复方蒲黄提取物
肾炎性血尿模型
蛋白尿
compound pollen typhae extract
glomerulonephritis hematuria model
proteinuria
