摘要
目的:探讨胎儿稽留流产与染色体非整倍体及其他致病因素的关系。方法:采用FISH技术对267例稽留流产胎儿标本进行13,16,18,21,22,X,Y染色体数目检测,利用荧光显微镜观察结果,采用SPSS13.0软件进行统计分析。结果:267例样本中,染色体数目异常95例,异常率35.6%,以三体最常见,其次为四体,少见部分单体;异常染色体存在混合嵌合现象,如混合嵌合三体(2n+1/2n),混合嵌合四体(2n+2/2n),混合嵌合单倍体、三体、四体(2n-1/2n+1/2n+2/2n)等;稽留流产与孕妇流产次数和孕周相关。结论:染色体数目异常及其混合嵌合均是导致稽留流产的重要原因,同时也与孕妇流产胎次、早期妊娠具有相关性。
Objective： To explore the relationship between missed abortion of fetuses and chromosomal aneuploidy and the other pathogenic factors. Methods： The numbers of chromosomes 13, 16, 18, 21,22, X, Y were detected by FISH technology in 267 specimens of fetal missed abortion, the results were observed under fluorescence microscope and analyzed statistically on SPSS13.0 software. Results： Among 267 specimens, 95 specimens were found with numerical abnormalities of chromosomes, accounting for 35.6% , trisomy was the most common type, followed by tetraploid, monosome was few ; mixed chimerism phenomenon was found in abnoimal chromosomes, such as mixed chimeric trisomy （2n ＋ 1/2n）, mixed chimeric tetraploid （2n ＋ 2/2n）, and mixed chimeric monosome/trisomy/tetraploid （2n- 1/2n ＋ 1/ 2n ＋ 2/2n） ; missed abortion was correlated with times and gestational weeks of abortion in pregnant women. Conclusion： Both numerical abnormalities of chromosomes and mixed chimerism are important causes of missed abortion, missed abortion is correlated with times of abor- tion and early pregnancy.
出处
《中国妇幼保健》
CAS
北大核心
2013年第33期5505-5507,共3页
Maternal and Child Health Care of China
基金
广西自然科学基金〔桂科自(0832182)〕
关键词
稽留流产
FISH技术
染色体非整倍体
多次流产早期妊娠
Missed abortion
FISH technology
Chromosomal aneuploidy
Multiple miscarriages
Early pregnancy
