摘要
样品经 HPLC分离后 ,利用氢化可的松对 Ce(IV) - SO-23 化学发光体系的增敏作用 ,在色谱柱为 C18氰基柱 ,水∶乙腈 80∶ 2 0 (体积分数 )为流动相的条件下 ,实现了对氢化可的松的分离与测定 ,方法的线性范围为 4× 1 0 -6～ 1×1 0 -3g/m L,检出限为 9× 1 0 -7g/m L,相对标准偏差 3.6% (n=1 1 )
In this paper,hydrocortisone was separated by HPLC and determined by chemiluminescence(CL) based on its enhancing effect in cerium(Ⅳ) sulphite reaction.The colum used was cyzno C 18 colum with pump rate of 1 2 mL/min.The linear range for hydrocortisone is 4×10 -6 ～1×10 -3 g/mL,the detection limit is 9×10 -7 g/mL,the relative standard deviation is 3 6% ( n =11).
出处
《分析试验室》
CAS
CSCD
2000年第6期30-32,共3页
Chinese Journal of Analysis Laboratory
基金
陕西省科委资助项目
