摘要
A new method for the determination of baicalin with HPLC-CL was developed. The method was based on the chemiluminescence reaction between KMnO4 and baicalin sensitized from HCHO. The linear range was 3.7?0-6~9.8?0-5 mol/L with detection limit of 1.7?0-6 mol/L and the relative standard deviation was 2.5 % (Cs=6.6?0-5 mol/L, n=5). The method has been applied to the determination of baicalin in oral administration, injection, Scutellariae radix and granules with good results.
A new method for the determination of baicalin with HPLC-CL was developed. The method was based on the chemiluminescence reaction between KMnO4 and baicalin sensitized from HCHO. The linear range was 3.T×10^6∽9.8×10^-5 mol/L with detection limit of 1.7×10^-6mol/L and the relative standard deviation was 2.5 % (Cs=6.6×10^-5 mol/L, n=5). The method has been applied to the determination of baicalin in oral administration, injection, Scutellariae radix and granules with good results.
