摘要
比较了径向离子交换色谱与经典离子交换色谱在纯化工艺放大过程中的分离效果 ,证实了径向色谱比经典色谱更适合于促卵泡激素 (FSH)的纯化 ,并建立了适合于大规模生产FSH的径向色谱纯化新工艺。纯化后FSH的比活性为 180IU/mg ,活性回收率为 5 6 % 。
By comparing the purification results of radial flow chromatography with conventional chromatography, a new purification process of follicle stimulating hormone (FSH) from crude sample of human menopausal gonadotrophin has been developed The results showed that the specific activity, activity recovery, and purification factor of FSH purified by radial flow chromatography were higher than those by conventional chromatography in large scale The specific activity of FSH purified was 180 IU/mg protein, activity recovery 56%, purification factor 150 The purified product meets the quality requirement of the Standard of Chinese Pharmacopoeia.
出处
《色谱》
CAS
CSCD
北大核心
2000年第6期577-579,共3页
Chinese Journal of Chromatography
基金
陕西省科学技术发展计划项目攻关课题! (95K11 8)
高等学校骨干教师资助计划资助
关键词
径向色谱
离子交换色谱
促卵泡激素
纯化工艺
radial flow chromatography
ion exchange chromatography
follicle stimulating hormone
purification
